LOCUS       BBa_K1913035            1102 bp    DNA     linear       25-Jun-2024
DEFINITION  Constitutive promoter with Guanine riboswitch BS-yxjA with mRFP
ACCESSION   BBa_K1913035
VERSION     BBa_K1913035.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     stem_loop       44..124
                     /label=G box
     misc_feature    123..129
                     /label=Anti-terminator
     misc_feature    131..137
                     /label=Anti-terminator
     stem_loop       172..232
                     /label=terminator
     misc_feature    180..187
                     /label=Anti-terminator
     misc_feature    189..195
                     /label=Anti-terminator
     misc_feature    44..232
                     /label=BBa_K1913019
     RBS             241..253
                     /label=RBS-3\Weak
     RBS             247..250
                     /label=RBS
     misc_feature    241..253
                     /label=BBa_B0032
     RBS             241..253
                     /label=BBa_B0032
     CDS             260..934
                     /label=mrfp1
     misc_feature    941..965
                     /label=Help:Barcodes
     CDS             260..965
                     /label=BBa_E1010
     stem_loop       985..1028
                     /label=stem_loop
     misc_feature    974..1053
                     /label=BBa_B0010
     terminator      974..1053
                     /label=BBa_B0010
     stem_loop       1069..1088
                     /label=T7 TE
     misc_feature    1095..1095
                     /label=stop
     polyA_site      1089..1102
                     /label=polya
     misc_feature    1062..1102
                     /label=BBa_B0012
     terminator      1062..1102
                     /label=BBa_B0012
     terminator      974..1102
                     /label=BBa_B0015
     misc_feature    241..1102
                     /label=BBa_K516032
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaggaaaa agacattctt
       61 gtatatgatc agtaatatgg tctgattgtt tctacctagt aaccgtaaaa aactagacta
      121 caagaaagtt tgaaaatttg aacgagttga aaaggacaag ttcttttctg tttgctctta
      181 tttttcacac tttctgcact tccagaattt gtgaaggata agagcttttt tttactagag
      241 tcacacagga aagtactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
      301 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      361 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      421 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      481 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      541 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      601 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      661 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
      721 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      781 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
      841 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
      901 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
      961 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1021 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1081 ggtgggcctt tctgcgttta ta
//