BBa_K1913035
type: ComponentDefinition
persistent identity: BBa K1913035
display id: BBa_K1913035
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive promoter with Guanine riboswitch BS-yxjA with mRFP
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T07:57:11Z
mutable provenance: B. subtilis and iGEM part registry
owned by: james
owned by: myers
mutable description: Measurement device includes constitutive promoter Guanine riboswitch BS-yxjA and mRFP reporter.The binding of metabolites (guanine molecule) to the riboswitches on the 5??? UTR of the mRFP mRNA will attenuate translation of mRFP
discontinued: false
dominant: false
group u list: _2380_
m user id: 29979
owner id: 29979
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Testing Guanine riboswitch BS-yxjA in vivo
star: false
creator: Tianhe Wang
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J23106
component: BBa_K516032
component: BBa_K1913019
sequence annotation: BBa_J23106
sequence annotation: BBa_K1913019
sequence annotation: BBa_K516032
sequence: https://synbiohub.org/public/igem/BBa_K1913035_sequence/1

BBa_J23106
type: Component
persistent identity: component2505644
display id: component2505644
version: 1
top level: BBa_K1913035
definition: BBa_J23106
access: public

BBa_K516032
type: Component
persistent identity: component2505659
display id: component2505659
version: 1
top level: BBa_K1913035
definition: BBa_K516032
access: public

BBa_K1913019
type: Component
persistent identity: component2505645
display id: component2505645
version: 1
top level: BBa_K1913035
definition: BBa_K1913019
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2505644
display id: annotation2505644
version: 1
top level: BBa_K1913035
location: https://synbiohub.org/public/igem/BBa_K1913035/annotation2505644/range2505644/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1913035/annotation2505644/range2505644/1
type: Range
persistent identity: range2505644
display id: range2505644
version: 1
top level: BBa_K1913035
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1913019
type: SequenceAnnotation
persistent identity: annotation2505645
display id: annotation2505645
version: 1
top level: BBa_K1913035
location: https://synbiohub.org/public/igem/BBa_K1913035/annotation2505645/range2505645/1
component: BBa_K1913019

https://synbiohub.org/public/igem/BBa_K1913035/annotation2505645/range2505645/1
type: Range
persistent identity: range2505645
display id: range2505645
version: 1
top level: BBa_K1913035
direction: direction/reverse
start: 44
end: 232
orientation: inline

BBa_K516032
type: SequenceAnnotation
persistent identity: annotation2505659
display id: annotation2505659
version: 1
top level: BBa_K1913035
location: https://synbiohub.org/public/igem/BBa_K1913035/annotation2505659/range2505659/1
component: BBa_K516032

https://synbiohub.org/public/igem/BBa_K1913035/annotation2505659/range2505659/1
type: Range
persistent identity: range2505659
display id: range2505659
version: 1
top level: BBa_K1913035
direction: direction/reverse
start: 241
end: 1102
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K1913019
type: ComponentDefinition
persistent identity: BBa K1913019
display id: BBa_K1913019
version: 1
was generated by: iGEM to SBOL conversion
description: Guanine riboswitch BS-yxjA
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T07:37:54Z
mutable provenance: Genomic sequence of B. subtilis
owned by: james
owned by: myers
mutable description: Guanine riboswitches BS-yxjA negatively modulate transcription upon guanine binding.BS-yxjA variant displays a higher 2AP affinity with an increase of 13-fold relative to other variants in vitro test.
discontinued: false
dominant: false
group u list: _2380_
m user id: 29979
owner id: 29979
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: According to Mulhbacher J et al, riboswitches BS-yxjA showed wide range sensitivity of Guanine concentration in vitro test.
star: false
creator: Tianhe Wang
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: G box
sequence annotation: Anti-terminator
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_K1913019_sequence/1

G box
type: SequenceAnnotation
persistent identity: annotation2505991
display id: annotation2505991
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2505991/range2505991/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1913019/annotation2505991/range2505991/1
type: Range
persistent identity: range2505991
display id: range2505991
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 1
end: 81
orientation: inline

Anti-terminator
type: SequenceAnnotation
persistent identity: annotation2505992
display id: annotation2505992
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2505992/range2505992/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1913019/annotation2505992/range2505992/1
type: Range
persistent identity: range2505992
display id: range2505992
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 80
end: 86
orientation: inline

Anti-terminator
type: SequenceAnnotation
persistent identity: annotation2505993
display id: annotation2505993
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2505993/range2505993/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1913019/annotation2505993/range2505993/1
type: Range
persistent identity: range2505993
display id: range2505993
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 88
end: 94
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2505994
display id: annotation2505994
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2505994/range2505994/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1913019/annotation2505994/range2505994/1
type: Range
persistent identity: range2505994
display id: range2505994
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 129
end: 189
orientation: inline

Anti-terminator
type: SequenceAnnotation
persistent identity: annotation2506007
display id: annotation2506007
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2506007/range2506007/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1913019/annotation2506007/range2506007/1
type: Range
persistent identity: range2506007
display id: range2506007
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 137
end: 144
orientation: inline

Anti-terminator
type: SequenceAnnotation
persistent identity: annotation2506009
display id: annotation2506009
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2506009/range2506009/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1913019/annotation2506009/range2506009/1
type: Range
persistent identity: range2506009
display id: range2506009
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 146
end: 152
orientation: inline

BBa_K516032
type: ComponentDefinition
persistent identity: BBa K516032
display id: BBa_K516032
version: 1
was generated by: iGEM to SBOL conversion
description: mRFP protein generator TERM+ RBS+(B0032)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: Registry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP protein generator TERM+ RBS+(B0032)
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: --
star: false
creator: Nicolo' Politi, Federica Sampietro, Davide Bianchini
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0015
component: BBa_E1010
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K516032_sequence/1

BBa_B0015
type: Component
persistent identity: component2250608
display id: component2250608
version: 1
top level: BBa_K516032
definition: BBa_B0015
access: public

BBa_E1010
type: Component
persistent identity: component2250601
display id: component2250601
version: 1
top level: BBa_K516032
definition: mRFP1
access: public

BBa_B0032
type: Component
persistent identity: component2250597
display id: component2250597
version: 1
top level: BBa_K516032
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250597
display id: annotation2250597
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250597/range2250597/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K516032/annotation2250597/range2250597/1
type: Range
persistent identity: range2250597
display id: range2250597
version: 1
top level: BBa_K516032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250601
display id: annotation2250601
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250601/range2250601/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K516032/annotation2250601/range2250601/1
type: Range
persistent identity: range2250601
display id: range2250601
version: 1
top level: BBa_K516032
direction: direction/reverse
start: 20
end: 725
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250608
display id: annotation2250608
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250608/range2250608/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516032/annotation2250608/range2250608/1
type: Range
persistent identity: range2250608
display id: range2250608
version: 1
top level: BBa_K516032
direction: direction/reverse
start: 734
end: 862
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1913035_sequence/1
type: Sequence
persistent identity: BBa K1913035 sequence
display id: BBa_K1913035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagctactagagaggaaaaagacattcttgtatatgatcagtaatatggtctgattgtttctacctagtaaccgtaaaaaactagactacaagaaagtttgaaaatttgaacgagttgaaaaggacaagttcttttctgtttgctcttatttttcacactttctgcacttccagaatttgtgaaggataagagcttttttttactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1913019_sequence/1
type: Sequence
persistent identity: BBa K1913019 sequence
display id: BBa_K1913019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaaaaagacattcttgtatatgatcagtaatatggtctgattgtttctacctagtaaccgtaaaaaactagactacaagaaagtttgaaaatttgaacgagttgaaaaggacaagttcttttctgtttgctcttatttttcacactttctgcacttccagaatttgtgaaggataagagcttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K516032_sequence/1
type: Sequence
persistent identity: BBa K516032 sequence
display id: BBa_K516032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z