Constitutive promoter with Guanine riboswitch BS-yxjA with mRFP
Measurement device includes constitutive promoter Guanine riboswitch BS-yxjA and mRFP reporter.The binding of metabolites (guanine molecule) to the riboswitches on the 5??? UTR of the mRFP mRNA will attenuate translation of mRFP
Testing Guanine riboswitch BS-yxjA in vivo
Guanine riboswitches BS-yxjA negatively modulate transcription upon guanine binding.BS-yxjA variant displays a higher 2AP affinity with an increase of 13-fold relative to other variants in vitro test.
According to Mulhbacher J et al, riboswitches BS-yxjA showed wide range sensitivity of Guanine concentration in vitro test.
mRFP protein generator TERM+ RBS+(B0032)
Nicolo' Politi, Federica Sampietro, Davide Bianchini
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
Double terminator consisting of BBa_B0010 and BBa_B0012
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1