LOCUS       BBa_K1914001             926 bp    DNA     linear       22-May-2024
DEFINITION  pT7- E. coli optimised - KillerOrange (EOKO)
ACCESSION   BBa_K1914001
VERSION     BBa_K1914001.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..789
                     /label=BBa_K1914000
     stem_loop       809..852
                     /label=stem_loop
     misc_feature    798..877
                     /label=BBa_B0010
     terminator      798..877
                     /label=BBa_B0010
     stem_loop       893..912
                     /label=T7 TE
     misc_feature    919..919
                     /label=stop
     polyA_site      913..926
                     /label=polya
     misc_feature    886..926
                     /label=BBa_B0012
     terminator      886..926
                     /label=BBa_B0012
     terminator      798..926
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag
       61 gagaaatact agatgatgga atgcggcccg gcgctgtttc agagcgatat gacctttaaa
      121 atttttattg atggcgaagt gaacggccag aaatttacca ttgtggcgga tggcagcagc
      181 aaatttccgc atggcgattt taacgtgcat gcggtgtgcg aaaccggcaa actgccgatg
      241 agctggaaac cgatttgcca tctgattcag tggggcgaac cgttttttgc gcgctatccg
      301 gatggcatta gccattttgc gcaggaatgc tttccggaag gcctgagcat tgatcgcacc
      361 gtgcgctttg aaaacgatgg caccatgacc agccatcata cctatgaact gagcgatacc
      421 tgcgtggtga gccgcattac cgtgaactgc gatggctttc agccggatgg cccgattatg
      481 cgcgatcagc tggtggatat tctgccgagc gaaacccata tgtttccgca tggcccgaac
      541 gcggtgcgcc agctggcgtt tattggcttt accaccgcgg atggcggcct gatgatgggc
      601 catctggata gcaaaatgac ctttaacggc agccgcgcga ttgaaattcc gggcccgcat
      661 tttgtgacca ttattaccaa acagatgcgc gataccagcg ataaacgcga tcatgtgtgc
      721 cagcgcgaag tggcgcatgc gcatagcgtg ccgcgcatta ccagcgcgat tggcagcgat
      781 caggattgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      901 ttcgggtggg cctttctgcg tttata
//