BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K1914000
1
BBa_K1914000
E. coli Codon Optimised KillerOrange(EOKO)
2016-10-06T11:00:00Z
2016-10-07T03:34:49Z
Mutant of KillerRed, a red fluorescent protein from jellyfish Anthomedusae sp chromoprotein anm2CP, produced by random mutatagenesis using an error-prone PCR.
Sarkisyan, K.S., Zlobovskaya, O.A., Gorbachev, D.A., Bozhanova, N.G., Sharonov, G.V., Staroverov, D.B., Egorov, E.S., Ryabova, A.V., Solntsev, K.M., Mishin, A.S. and Lukyanov, K.A., 2015. KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. PloS one,10(12), p.e0145287.
KillerOrange is a photoactiated kill switch codon optimised for E.coli optimised coding region of KillerRed mutant.
false
false
_2381_
31076
31076
9
false
Codon optimised for E.coli K12 using IDT's online server
false
Eloise Lloyd
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1914001
1
BBa_K1914001
pT7- E. coli optimised - KillerOrange (EOKO)
2016-10-06T11:00:00Z
2016-10-07T03:40:19Z
Mutant of KillerRed, a red fluorescent protein from jellyfish Anthomedusae sp chromoprotein anm2CP, produced by random mutatagenesis using an error-prone PCR.
Sarkisyan, K.S., Zlobovskaya, O.A., Gorbachev, D.A., Bozhanova, N.G., Sharonov, G.V., Staroverov, D.B., Egorov, E.S., Ryabova, A.V., Solntsev, K.M., Mishin, A.S. and Lukyanov, K.A., 2015. KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. PloS one,10(12), p.e0145287.
KillerOrange is a photoactiated kill switch with an IPTG inducible T7 promoter with E.coli optimised protein coding region of KillerRed mutant.
false
false
_2381_
31076
31076
9
false
Codon optimised for E.coli K12 using IDT's online server
false
Eloise Lloyd
component2486851
1
BBa_K1914000
component2486848
1
BBa_I712074
component2486850
1
BBa_B0034
component2486858
1
BBa_B0015
annotation2486848
1
BBa_I712074
range2486848
1
1
46
annotation2486850
1
BBa_B0034
range2486850
1
55
66
annotation2486851
1
BBa_K1914000
range2486851
1
73
789
annotation2486858
1
BBa_B0015
range2486858
1
798
926
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1914001_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatgatggaatgcggcccggcgctgtttcagagcgatatgacctttaaaatttttattgatggcgaagtgaacggccagaaatttaccattgtggcggatggcagcagcaaatttccgcatggcgattttaacgtgcatgcggtgtgcgaaaccggcaaactgccgatgagctggaaaccgatttgccatctgattcagtggggcgaaccgttttttgcgcgctatccggatggcattagccattttgcgcaggaatgctttccggaaggcctgagcattgatcgcaccgtgcgctttgaaaacgatggcaccatgaccagccatcatacctatgaactgagcgatacctgcgtggtgagccgcattaccgtgaactgcgatggctttcagccggatggcccgattatgcgcgatcagctggtggatattctgccgagcgaaacccatatgtttccgcatggcccgaacgcggtgcgccagctggcgtttattggctttaccaccgcggatggcggcctgatgatgggccatctggatagcaaaatgacctttaacggcagccgcgcgattgaaattccgggcccgcattttgtgaccattattaccaaacagatgcgcgataccagcgataaacgcgatcatgtgtgccagcgcgaagtggcgcatgcgcatagcgtgccgcgcattaccagcgcgattggcagcgatcaggattgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1914000_sequence
1
atgatggaatgcggcccggcgctgtttcagagcgatatgacctttaaaatttttattgatggcgaagtgaacggccagaaatttaccattgtggcggatggcagcagcaaatttccgcatggcgattttaacgtgcatgcggtgtgcgaaaccggcaaactgccgatgagctggaaaccgatttgccatctgattcagtggggcgaaccgttttttgcgcgctatccggatggcattagccattttgcgcaggaatgctttccggaaggcctgagcattgatcgcaccgtgcgctttgaaaacgatggcaccatgaccagccatcatacctatgaactgagcgatacctgcgtggtgagccgcattaccgtgaactgcgatggctttcagccggatggcccgattatgcgcgatcagctggtggatattctgccgagcgaaacccatatgtttccgcatggcccgaacgcggtgcgccagctggcgtttattggctttaccaccgcggatggcggcctgatgatgggccatctggatagcaaaatgacctttaacggcagccgcgcgattgaaattccgggcccgcattttgtgaccattattaccaaacagatgcgcgataccagcgataaacgcgatcatgtgtgccagcgcgaagtggcgcatgcgcatagcgtgccgcgcattaccagcgcgattggcagcgatcaggattga
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z