BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1914000 1 BBa_K1914000 E. coli Codon Optimised KillerOrange(EOKO) 2016-10-06T11:00:00Z 2016-10-07T03:34:49Z Mutant of KillerRed, a red fluorescent protein from jellyfish Anthomedusae sp chromoprotein anm2CP, produced by random mutatagenesis using an error-prone PCR. Sarkisyan, K.S., Zlobovskaya, O.A., Gorbachev, D.A., Bozhanova, N.G., Sharonov, G.V., Staroverov, D.B., Egorov, E.S., Ryabova, A.V., Solntsev, K.M., Mishin, A.S. and Lukyanov, K.A., 2015. KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. PloS one,10(12), p.e0145287. KillerOrange is a photoactiated kill switch codon optimised for E.coli optimised coding region of KillerRed mutant. false false _2381_ 31076 31076 9 false Codon optimised for E.coli K12 using IDT's online server false Eloise Lloyd BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1914001 1 BBa_K1914001 pT7- E. coli optimised - KillerOrange (EOKO) 2016-10-06T11:00:00Z 2016-10-07T03:40:19Z Mutant of KillerRed, a red fluorescent protein from jellyfish Anthomedusae sp chromoprotein anm2CP, produced by random mutatagenesis using an error-prone PCR. Sarkisyan, K.S., Zlobovskaya, O.A., Gorbachev, D.A., Bozhanova, N.G., Sharonov, G.V., Staroverov, D.B., Egorov, E.S., Ryabova, A.V., Solntsev, K.M., Mishin, A.S. and Lukyanov, K.A., 2015. KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. PloS one,10(12), p.e0145287. KillerOrange is a photoactiated kill switch with an IPTG inducible T7 promoter with E.coli optimised protein coding region of KillerRed mutant. false false _2381_ 31076 31076 9 false Codon optimised for E.coli K12 using IDT's online server false Eloise Lloyd component2486851 1 BBa_K1914000 component2486848 1 BBa_I712074 component2486850 1 BBa_B0034 component2486858 1 BBa_B0015 annotation2486848 1 BBa_I712074 range2486848 1 1 46 annotation2486850 1 BBa_B0034 range2486850 1 55 66 annotation2486851 1 BBa_K1914000 range2486851 1 73 789 annotation2486858 1 BBa_B0015 range2486858 1 798 926 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1914001_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatgatggaatgcggcccggcgctgtttcagagcgatatgacctttaaaatttttattgatggcgaagtgaacggccagaaatttaccattgtggcggatggcagcagcaaatttccgcatggcgattttaacgtgcatgcggtgtgcgaaaccggcaaactgccgatgagctggaaaccgatttgccatctgattcagtggggcgaaccgttttttgcgcgctatccggatggcattagccattttgcgcaggaatgctttccggaaggcctgagcattgatcgcaccgtgcgctttgaaaacgatggcaccatgaccagccatcatacctatgaactgagcgatacctgcgtggtgagccgcattaccgtgaactgcgatggctttcagccggatggcccgattatgcgcgatcagctggtggatattctgccgagcgaaacccatatgtttccgcatggcccgaacgcggtgcgccagctggcgtttattggctttaccaccgcggatggcggcctgatgatgggccatctggatagcaaaatgacctttaacggcagccgcgcgattgaaattccgggcccgcattttgtgaccattattaccaaacagatgcgcgataccagcgataaacgcgatcatgtgtgccagcgcgaagtggcgcatgcgcatagcgtgccgcgcattaccagcgcgattggcagcgatcaggattgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1914000_sequence 1 atgatggaatgcggcccggcgctgtttcagagcgatatgacctttaaaatttttattgatggcgaagtgaacggccagaaatttaccattgtggcggatggcagcagcaaatttccgcatggcgattttaacgtgcatgcggtgtgcgaaaccggcaaactgccgatgagctggaaaccgatttgccatctgattcagtggggcgaaccgttttttgcgcgctatccggatggcattagccattttgcgcaggaatgctttccggaaggcctgagcattgatcgcaccgtgcgctttgaaaacgatggcaccatgaccagccatcatacctatgaactgagcgatacctgcgtggtgagccgcattaccgtgaactgcgatggctttcagccggatggcccgattatgcgcgatcagctggtggatattctgccgagcgaaacccatatgtttccgcatggcccgaacgcggtgcgccagctggcgtttattggctttaccaccgcggatggcggcctgatgatgggccatctggatagcaaaatgacctttaacggcagccgcgcgattgaaattccgggcccgcattttgtgaccattattaccaaacagatgcgcgataccagcgataaacgcgatcatgtgtgccagcgcgaagtggcgcatgcgcatagcgtgccgcgcattaccagcgcgattggcagcgatcaggattga BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z