LOCUS       BBa_K1914003             929 bp    DNA     linear       22-Nov-2024
DEFINITION  pT7- E. coli optimised - KillerRed (EOKR)
ACCESSION   BBa_K1914003
VERSION     BBa_K1914003.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..792
                     /label=BBa_K1914002
     stem_loop       812..855
                     /label=stem_loop
     misc_feature    801..880
                     /label=BBa_B0010
     terminator      801..880
                     /label=BBa_B0010
     stem_loop       896..915
                     /label=T7 TE
     misc_feature    922..922
                     /label=stop
     polyA_site      916..929
                     /label=polya
     misc_feature    889..929
                     /label=BBa_B0012
     terminator      889..929
                     /label=BBa_B0012
     terminator      801..929
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag
       61 gagaaatact agatgggcag tgaaggtggt cctgcgcttt tccagtcaga catgaccttc
      121 aaaattttca ttgacggtga agttaatgga cagaaattta cgatcgtagc cgatggctca
      181 agcaaattcc cacatgggga cttcaatgtc cacgccgtgt gcgaaacagg caaattaccc
      241 atgagctgga agccgatttg tcatttgatt cagtacgggg agcctttttt cgctcgttac
      301 ccagatggaa tttctcactt tgcccaggag tgttttcccg aaggactgtc tatcgatcgt
      361 accgtgcgct ttgaaaacga cggtactatg acctcgcatc atacctatga attagacgat
      421 acatgcgtgg taagtcgtat cacggtaaac tgcgacggtt ttcaacctga tggcccaatc
      481 atgcgtgacc agttggtcga tatcctgcct aatgaaaccc atatgttccc gcatgggcca
      541 aatgcggtcc gccaattagc attcatcggg ttcacgactg cggacggcgg acttatgatg
      601 gggcattttg actctaagat gacctttaac ggttcgcgcg cgattgaaat tcctgggccg
      661 cactttgtga cgattattac aaagcaaatg cgtgatacat ctgacaaacg cgaccacgtc
      721 tgtcaacgtg aagtcgctta cgcacattca gtgcctcgca ttaccagtgc gatcggttca
      781 gatgaggact gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      841 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      901 accttcgggt gggcctttct gcgtttata
//