LOCUS       BBa_K1914005             686 bp    DNA     linear       26-Nov-2024
DEFINITION  pT7 Lysozyme C E.coli codon optimised, signal peptide, flag tag
ACCESSION   BBa_K1914005
VERSION     BBa_K1914005.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..549
                     /label=BBa_K1914004
     stem_loop       569..612
                     /label=stem_loop
     misc_feature    558..637
                     /label=BBa_B0010
     terminator      558..637
                     /label=BBa_B0010
     stem_loop       653..672
                     /label=T7 TE
     misc_feature    679..679
                     /label=stop
     polyA_site      673..686
                     /label=polya
     misc_feature    646..686
                     /label=BBa_B0012
     terminator      646..686
                     /label=BBa_B0012
     terminator      558..686
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag
       61 gagaaatact agatgaagaa gaccgcgatt gccatcgctg ttgctcttgc tggcttcgct
      121 acggtcgcgc aggctgacta taaagacgat gatgataaaa aagtcttcgg tcgctgtgag
      181 cttgccgcag ccatgaagcg ccacgggctt gacaactacc gtggatactc attaggaaac
      241 tgggtctgcg ctgctaaatt cgaatctaac ttcaatacgc aagcgaccaa tcgtaacaca
      301 gatggatcga cagactatgg tattttgcaa attaacagtc gctggtggtg taatgatggc
      361 cgcacacccg gatcacgtaa tctgtgtaat atcccatgct ctgcgctttt atctagcgac
      421 atcaccgctt ccgtgaactg cgctaagaag attgtgtcag acggaaacgg aatgaacgct
      481 tgggttgctt ggcgtaatcg ttgtaaaggc actgatgtcc aagcctggat tcgcggatgc
      541 cgcctgtaat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      601 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      661 ttcgggtggg cctttctgcg tttata
//