BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1914005 1 BBa_K1914005 pT7 Lysozyme C E.coli codon optimised, signal peptide, flag tag 2016-10-06T11:00:00Z 2016-10-07T05:15:27Z Lysozyme C Gallus gallus Part:BBa_K284001 http://parts.igem.org/Part:BBa_K284001 Lysozyme C from Gallus gallus codon optomised for E.coli with a OmpA signal peptide to target the periplasm as well as a flag tag. The device is under a T7 promoter iducible using IPTG. false false _2381_ 31076 31076 9 false E.coli codon optomised using IDT programme, OmpA signal peptide and flag tag false Eloise Lloyd component2486989 1 BBa_B0015 component2486981 1 BBa_B0034 component2486979 1 BBa_I712074 component2486982 1 BBa_K1914004 annotation2486979 1 BBa_I712074 range2486979 1 1 46 annotation2486989 1 BBa_B0015 range2486989 1 558 686 annotation2486982 1 BBa_K1914004 range2486982 1 73 549 annotation2486981 1 BBa_B0034 range2486981 1 55 66 BBa_K1914004 1 BBa_K1914004 Lysozyme C E.coli codon optimised, signal peptide, flag tag 2016-10-06T11:00:00Z 2016-10-07T05:08:53Z Lysozyme from Gallus gallus Part:BBa_K284001 http://parts.igem.org/Part:BBa_K284001 Lysozyme C from Gallus gallus, codon optimised for E.coli using IDT website programme. A flag tag was added as well as an OmpA signal peptide amino acid sequence came from E. coli and targets the lysozyme to the periplasm. The device is under a T7 IPTG inducible promoter. false false _2381_ 31076 31076 9 false E.coli optimisation using IDT website programme, OmpA signal peptide targeting to the periplasm, flag tag false Eloise Lloyd BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_K1914005_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatgaagaagaccgcgattgccatcgctgttgctcttgctggcttcgctacggtcgcgcaggctgactataaagacgatgatgataaaaaagtcttcggtcgctgtgagcttgccgcagccatgaagcgccacgggcttgacaactaccgtggatactcattaggaaactgggtctgcgctgctaaattcgaatctaacttcaatacgcaagcgaccaatcgtaacacagatggatcgacagactatggtattttgcaaattaacagtcgctggtggtgtaatgatggccgcacacccggatcacgtaatctgtgtaatatcccatgctctgcgcttttatctagcgacatcaccgcttccgtgaactgcgctaagaagattgtgtcagacggaaacggaatgaacgcttgggttgcttggcgtaatcgttgtaaaggcactgatgtccaagcctggattcgcggatgccgcctgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1914004_sequence 1 atgaagaagaccgcgattgccatcgctgttgctcttgctggcttcgctacggtcgcgcaggctgactataaagacgatgatgataaaaaagtcttcggtcgctgtgagcttgccgcagccatgaagcgccacgggcttgacaactaccgtggatactcattaggaaactgggtctgcgctgctaaattcgaatctaacttcaatacgcaagcgaccaatcgtaacacagatggatcgacagactatggtattttgcaaattaacagtcgctggtggtgtaatgatggccgcacacccggatcacgtaatctgtgtaatatcccatgctctgcgcttttatctagcgacatcaccgcttccgtgaactgcgctaagaagattgtgtcagacggaaacggaatgaacgcttgggttgcttggcgtaatcgttgtaaaggcactgatgtccaagcctggattcgcggatgccgcctgtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z