BBa_J61101
1
BBa_J61101
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
fix
false
false
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2000
1
-35
range2000
1
20
25
annotation1999
1
lac O1
range1999
1
3
19
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2001
1
lac O1
range2001
1
26
42
annotation2002
1
-10
range2002
1
43
48
BBa_K1915998
1
BBa_K1915998
LTNF Scar 2
2016-10-17T11:00:00Z
2016-10-18T06:22:12Z
NA
LTNF Scar from IDT
false
false
_2382_
16948
16948
9
false
NA
false
Jessica Siemer
BBa_K1915003
1
BBa_K1915003
Inducible Lac promoter+LTNF15
2016-10-13T11:00:00Z
2016-10-18T07:00:49Z
This is a composite part that was assembled through gene synthesis from IDT.
This is a generator part for BBa_K1915002. This part includes an IPTG inducible promoter, RBS J61101, and the LTNF15 peptide coding sequence.
false
false
_2382_
16948
33829
9
false
This composite part was synthesized as a whole unit by IDT. Therefore there are no Biobrick scars. Instead a spacer "TCATAGAG" was included following R0011 and "TACTAGATG" following J61101 to improve translation.
false
Tatenda Tela
component2519947
1
BBa_K1915999
component2519942
1
BBa_R0011
component2519949
1
BBa_K1915998
component2519955
1
BBa_K1915002
component2519948
1
BBa_J61101
annotation2519942
1
BBa_R0011
range2519942
1
1
54
annotation2519948
1
BBa_J61101
range2519948
1
64
75
annotation2519949
1
BBa_K1915998
range2519949
1
76
84
annotation2519955
1
BBa_K1915002
range2519955
1
85
219
annotation2519947
1
BBa_K1915999
range2519947
1
56
63
BBa_K1915999
1
BBa_K1915999
LTNF Scar1
2016-10-17T11:00:00Z
2016-10-18T07:10:11Z
NA
Scar from IDT part
false
false
_2382_
16948
16948
9
false
NA
false
Jessica Siemer
BBa_K1915002
1
BBa_K1915002
LTNF15
2016-10-13T11:00:00Z
2016-10-14T11:08:34Z
The sequence for the LTNF peptides were obtained from (G Chavan, et. al. (2014). Protein and peptide letters)
The full sequence was synthesized by IDT.
LTNF15 (Lethal Toxin Neutralizing Factor) is a 10 residue peptide that has been shown to neutralize toxins from snakes, scorpions, and bacteria. LTNF peptides were first isolated from opossum (Didephis virginiana) serum. This part contains the LTNF15 peptide as well as C-term Myc and 6xHis tags that can be used for purification and then later removed using the HRV (Human Rhinovirus) protease.
false
false
_2382_
33829
33829
9
false
N/A
false
Tatenda Tela
annotation2507148
1
HRV 3C site
range2507148
1
46
69
annotation2507124
1
LTNF15
range2507124
1
1
45
annotation2507187
1
stop codon
range2507187
1
133
135
annotation2507150
1
Myc tag
range2507150
1
70
99
annotation2507151
1
6 His
range2507151
1
115
132
BBa_K1915002_sequence
1
ctgaaagcgatggatccgaccccgccgctgtggattaaaaccgaactggaagtgctgtttcagggcccggaacagaaactgattagcgaagaagatctgaccagcgcggtggatcatcaccatcatcaccattaa
BBa_J61101_sequence
1
aaagacaggacc
BBa_K1915003_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatcatagagaaagacaggacctactagatgctgaaagcgatggatccgaccccgccgctgtggattaaaaccgaactggaagtgctgtttcagggcccggaacagaaactgattagcgaagaagatctgaccagcgcggtggatcatcaccatcatcaccattaa
BBa_K1915998_sequence
1
tactagatg
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
BBa_K1915999_sequence
1
tcatagagg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z