BBa_J61101 1 BBa_J61101 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A fix false false _95_ 0 483 95 In stock false N/A true John Anderson BBa_K1800003 1 BBa_K1800003 CBDA Synthase 2015-08-27T11:00:00Z 2015-08-28T12:05:49Z Synthesized by IDT CBDA Synthase (Cannabidiolic acid synthase) is an enzyme that catalyzes the conversion of cannabigerolic-acid into CBDA. false false _2225_ 27447 27447 9 false Assembly Standard 10 false Laura Lee Irvin BBa_K1915004 1 BBa_K1915004 CBDAs with Promoter 2016-10-13T11:00:00Z 2016-10-19T12:19:59Z TBD TBD false false _2382_ 33827 33829 9 false TBD false Kayla Ginn component2521808 1 BBa_J61101 component2521803 1 BBa_R0011 component2521809 1 BBa_K1800003 annotation2521808 1 BBa_J61101 range2521808 1 64 75 annotation2521809 1 BBa_K1800003 range2521809 1 82 1716 annotation2521803 1 BBa_R0011 range2521803 1 1 54 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation2002 1 -10 range2002 1 43 48 BBa_J61101_sequence 1 aaagacaggacc BBa_K1915004_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagacaggacctactagatgaagtgcagcacattttccttctggttcgtgtgcaagattattttctttttcttctccttcaatatccagacaagcatcgctaatccaagggaaaacttcctgaagtgtttttctcagtacatccccaacaatgctacaaacttgaagctggtgtatacccaaaataatcctctctacatgtcagtgttgaactccacaattcataacttgaggtttacttcagatactaccccaaaacctctagttatcgtgactccttcccacgttagtcacattcaaggtactattttatgttcaaagaaggtgggattgcagatccgtaccaggtcagggggccatgactctgagggcatgagttacattagccaggtccccttcgtcatcgtggatcttagaaacatgaggtctatcaagatcgatgtgcattctcagactgcatgggtcgaggcaggcgctaccctcggcgaggtctactactgggttaacgagaagaacgagaacttaagcttggccgcaggctattgccccaccgtctgcgccggcgggcacttcggcggtgggggatacggtccattgatgagaaattatggtcttgcagccgacaacattatcgatgcacacttagtcaacgttcacggaaaggtgttggaccgtaagtccatgggggaggatctgttctgggccctgaggggagggggagcagagtctttcggtattatagttgcttggaaaattaggcttgtggcagttcctaagagtacaatgtttagcgtcaagaagatcatggagattcacgagcttgtcaagcttgtgaataagtggcagaatatagcttacaagtacgacaaagaccttttgcttatgacacacttcatcaccaggaacatcacagataatcagggtaaaaacaagacagctatccatacatacttcagttctgtctttttgggaggagtggactccctcgtggatttgatgaataagtctttcccggagcttggtatcaagaagacagactgccgacagttatcttggatcgataccattatcttctactccggtgtggttaattacgacaccgataacttcaacaaggaaatcctcttggacaggtcagccggccagaacggggcctttaagatcaagttggactacgtgaagaagcctattccagagtcagtcttcgttcagatccttgagaagctttatgaggaggatatcggggctggtatgtacgctctttacccctacggaggaatcatggacgagatctctgagtccgcaatcccattccctcaccgagctggaatcctttatgaactttggtacatctgtagttgggagaagcaagaggacaatgagaagcacttgaactggatcagaaacatctataactttatgaccccctacgtgtcaaagaaccccaggttggcatacttgaactacagggacttggatatcggcattaacgaccccaagaatcccaacaactatacccaggcaaggatttggggcgagaagtacttcggcaagaactttgacaggctcgtgaaggtgaagaccctggttgatcccaacaatttcttcaggaacgagcagtcaattcctcctcttccgaggcataggcattga BBa_K1800003_sequence 1 atgaagtgcagcacattttccttctggttcgtgtgcaagattattttctttttcttctccttcaatatccagacaagcatcgctaatccaagggaaaacttcctgaagtgtttttctcagtacatccccaacaatgctacaaacttgaagctggtgtatacccaaaataatcctctctacatgtcagtgttgaactccacaattcataacttgaggtttacttcagatactaccccaaaacctctagttatcgtgactccttcccacgttagtcacattcaaggtactattttatgttcaaagaaggtgggattgcagatccgtaccaggtcagggggccatgactctgagggcatgagttacattagccaggtccccttcgtcatcgtggatcttagaaacatgaggtctatcaagatcgatgtgcattctcagactgcatgggtcgaggcaggcgctaccctcggcgaggtctactactgggttaacgagaagaacgagaacttaagcttggccgcaggctattgccccaccgtctgcgccggcgggcacttcggcggtgggggatacggtccattgatgagaaattatggtcttgcagccgacaacattatcgatgcacacttagtcaacgttcacggaaaggtgttggaccgtaagtccatgggggaggatctgttctgggccctgaggggagggggagcagagtctttcggtattatagttgcttggaaaattaggcttgtggcagttcctaagagtacaatgtttagcgtcaagaagatcatggagattcacgagcttgtcaagcttgtgaataagtggcagaatatagcttacaagtacgacaaagaccttttgcttatgacacacttcatcaccaggaacatcacagataatcagggtaaaaacaagacagctatccatacatacttcagttctgtctttttgggaggagtggactccctcgtggatttgatgaataagtctttcccggagcttggtatcaagaagacagactgccgacagttatcttggatcgataccattatcttctactccggtgtggttaattacgacaccgataacttcaacaaggaaatcctcttggacaggtcagccggccagaacggggcctttaagatcaagttggactacgtgaagaagcctattccagagtcagtcttcgttcagatccttgagaagctttatgaggaggatatcggggctggtatgtacgctctttacccctacggaggaatcatggacgagatctctgagtccgcaatcccattccctcaccgagctggaatcctttatgaactttggtacatctgtagttgggagaagcaagaggacaatgagaagcacttgaactggatcagaaacatctataactttatgaccccctacgtgtcaaagaaccccaggttggcatacttgaactacagggacttggatatcggcattaacgaccccaagaatcccaacaactatacccaggcaaggatttggggcgagaagtacttcggcaagaactttgacaggctcgtgaaggtgaagaccctggttgatcccaacaatttcttcaggaacgagcagtcaattcctcctcttccgaggcataggcattga BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z