BBa_K1916000
1
BBa_K1916000
NpF2164g5 + intein-CBD tag
2016-10-08T11:00:00Z
2016-10-09T10:33:56Z
From cyanobacteria genome
A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain attached to the C-terminus for purification. As with all CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and appears vibrant blue-green under normal light.
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2489551
1
intein/chitin binding domain
range2489551
1
514
1296
annotation2489549
1
NpF2164g5
range2489549
1
1
513
BBa_K1916000_sequence
1
atggaagaacaagtgctgacagcgatggtggaaaaaatccgtcaagcacaggatatggacacaattttcctgaatgtactgccaagtttacgcaaacaactacagtgcgatcgcctagcagtatttcgctttcatcccgactggagtgtggagtttgttgccgaatccgtaaaagataaatggttgtctttagctgattccgacatcaaaacgatttggatggacgaacatttacaagaaactcagggcggacgctatcgcaatcatgaaacctttgttgtcaatgatatctacactgtcggtcacgttcaatgctacctagagatattagaaaaaatccaggctaaggcttacgcgatcgctccgatctttattggaaacaaactctggggctttatcggagcataccaaaatactggccctcgcgagtggaaatccaaggatgtgcaactgttgcgaaaagtagctgtgcaaatgggtataggcttgcaacaggttaagtatatcgaacagtgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z