BBa_K1916001 1 BBa_K1916001 NpF2164g5 + intein-CBD tag 2016-10-09T11:00:00Z 2016-10-09T11:05:22Z Cyanobacteria genome A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain attached to the C-terminus for purification. As with all CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and appears vibrant blue-green under normal light. RFC10 compatible. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2489578 1 NpF2164g5 range2489578 1 1 513 annotation2489579 1 intein/chitin binding domain range2489579 1 514 1296 BBa_K1916001_sequence 1 atggaagaacaagtgctgacagcgatggtggaaaaaatccgtcaagcacaggatatggacacaattttcctgaatgtactgccaagtttacgcaaacaactacagtgcgatcgcctagcagtatttcgctttcatcccgactggagtgtggagtttgttgccgaatccgtaaaagataaatggttgtctttagctgattccgacatcaaaacgatttggatggacgaacatttacaagaaactcagggcggacgctatcgcaatcatgaaacctttgttgtcaatgatatctacactgtcggtcacgttcaatgctacctagagatattagaaaaaatccaggctaaggcttacgcgatcgctccgatctttattggaaacaaactctggggctttatcggagcataccaaaatactggccctcgcgagtggaaatccaaggatgtgcaactgttgcgaaaagtagctgtgcaaatgggtataggcttgcaacaggttaagtatatcgaacagtgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z