BBa_K1916002 1 BBa_K1916002 Cyan7427_1390g3 + intein-CBD tag 2016-10-09T11:00:00Z 2016-10-10T09:35:16Z Cyanobacteria genome A GAF domain of the cyanobacteriochrome (CBCR) Cyan7427_1390 with an intein/chitin-binding domain attached to the C-terminus for purification. As with all CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2491656 1 Cyan7427_1390g3 range2491656 1 1 582 annotation2491657 1 intein/chitin binding domain range2491657 1 583 1365 BBa_K1916002_sequence 1 atggaacaacaactacgacagcaaaaaaatctgggtacaatcattcaacacatccgtgaatccctcgatctgcaacaaattttggccacggtgacccagcaggttaaagaagccctccagggtgatcgggtgatcgtatttcaactgtttcccaatggtaaaagtcggattgtggaggaagcggtatccagtggattgacggtcctgaaagcaggccactgggaagatgaagtctggccgcaagagattctcgattactactggcaaggccaaccccggatcgttgctgatgtcatggatgataggtggacagattgtttagttgggtattctaaacaaggagaaattgtctctaagatcgttgccccgattctgcaagatatccatacttttgaggaaaatccctgggccaacccatccaagcgacatcaactttggggggtgctcgtcattcatgcctgccgccaaccgcgggtttggaaagcagaagaagcccagttactccagcaaattgccaaccagttagcgatcgctatccaacaggccaatctgtttgagcaactccagcagcccggggatccgtgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z