BBa_K1916006
1
BBa_K1916006
Ga0039498_104087g2 + intein-CBD tag
2016-10-10T11:00:00Z
2016-10-11T01:42:36Z
Cyanobacteria genome
A GAF domain of the cyanobacteriochrome (CBCR) Ga0039498_104087 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009).
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2491763
1
intein/chitin binding domain
range2491763
1
583
1365
annotation2491762
1
Ga0039498_104087g2
range2491762
1
1
582
BBa_K1916006_sequence
1
atggaacagcaattgcgagaacagcaaaccttagcgacaatcaccaataaaattcgcgaatcacttagtatcaaagagattctggcagtggtgactcaacaggttaaagatatgctgtctggcgatcgcgcgatcatctttcaactatttgataatggtaacagccaaattgtcgaagaatctgtacatagtaactttctcaatctcaaagcccttaattgggacaacgaagtttggtcacaggaaattctcgattgctattggcaaggcaagcctcgcattgttcccgatgtgatgaatgacatttggacagaatgcctagtggaatactcgctcaaaggtcagatcaagtccaaaattgtcgcgccaattctcctagagtcgcacattagcgaaaatcatcgctgggtagcaacggacggatataaaaagctgtggggtgttttagtagtccatgcctgtgcagaacagcgagaatggcaagattcagaagctcaactgctccaacaaattgcgaatcaattagcgatcgctattcagcaagctaatctatatgagcaatctcaacaacccggggatccgtgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z