BBa_K1916008 1 BBa_K1916008 Osc7112_5903g3 + intein-CBD tag 2016-10-10T11:00:00Z 2016-10-11T08:57:20Z Cyanobacteria genome A GAF domain of the cyanobacteriochrome (CBCR) Osc7112_5903 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2493107 1 osc7112_5903g3 range2493107 1 1 642 annotation2493109 1 intein/chitin binding domain range2493109 1 643 1425 BBa_K1916008_sequence 1 atggaacaatcagaactggctatccgcaagcaaacagaaaaggcgatcgctttacaactgcaacggcagcgaactttgggagaaatcgcccagcaaattcgcgaatcgttggatattaacgaaattttggcgactgtgacacagcaagtcaaagaaatcttgcaaggcgatcggatcgtggtgttccggctatttggcgatggcagaagtcaaattgttgaagaagctgtgtctagcgagttccccgcgctcaaagaccatcactgggaggacgaactgtggtcgccagaaatcctcaaccggtactggcagggcaagccccgcatcgtccccgatgtcatgaccgatatctggactgattgcttggtggaatacgcaaccgtttgtcaagtccagtccaaaatcgttgcacctatcctgcaagaagtccgaagcagcgaaagccatagatgggttgccccagggcaaaccaagaagctctggggggttttagtagtccacgcctgtcgagaacaacgagtatggcaagagtcggaagcgcaactcctgcaacaaattgccaaccagttggcgatcgcaattcaacaagctagcctatttaagcagttgcagcaagaactcaccgaacggcccggggatccgtgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z