BBa_K1916010
1
BBa_K1916010
CBCR2 + intein-CBD tag
2016-10-10T11:00:00Z
2016-10-11T09:59:07Z
Metagenomic data mining (JGI)
Fusion protein consisting of the GAF domain of an uncharacterized cyanobacteriochrome (CBCR) sequence and an intein/chitin-binding domain attached to the C-terminus for purification. Labelled as CBCR2.
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2493120
1
intein/chitin binding domain
range2493120
1
514
1296
annotation2493115
1
CBCR2
range2493115
1
1
513
BBa_K1916010_sequence
1
atggagcaagagcgactgctgcggctcattgtccaaaatattcatcaatcgctcaatctagacgaaatcctggcgacgatgctagacgaaacacggcagacgctccaggttgatcgggtaacggtttaccagtttgaacccgactggagtggtcgatttgtcgccgagtcggtcggggaagaatggatgcctctggtcgcggagggtgtgcagacggtttgggaagatacctatttgcaagacacccaagacgggcgctatcgccagaacgaaacctttgccgtcagcgatatttatcaggccgggcataccgactgccacattgaaattctggagcaatttcaggtgcgggcctatgcgatcgcccccatttttctggacgaacagctttgggggttactggcgatttatcaaaacacgggcccacgagactggcaagcctgggaactcgaactgctgcaacagatcagcctgcaaacggcgatcgccctgcgtcaatccaacctgtaccaatgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z