BBa_K1916015 1 BBa_K1916015 CBCR4 + intein-CBD tag 2016-10-10T11:00:00Z 2016-10-11T10:14:18Z Metagenomic data mining (JGI) Fusion protein consisting of the GAF domain of an uncharacterized cyanobacteriochrome (CBCR) sequence and an intein/chitin-binding domain attached to the C-terminus for purification. Labelled as CBCR4. RFC10 compatible. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2493135 1 intein/chitin binding domain range2493135 1 502 1284 annotation2493133 1 CBCR4 range2493133 1 1 501 BBa_K1916015_sequence 1 atgcgtgaaaagctgcttaacttaattacacagaacattcgtcagagtttggatctggaccagatattgcaaactactgttgatgaggtacgtaactttttagaaacggacagagtcattatatatcgtttgaaccctgactggtcaggcgtagtgatcacagaatcggttagcgaagaaagtctgtccattttagggatggagataactgacgcttacttcgttgagactaaaggcggtaattaccacaatggtaagattaataatatctccgacatctataaggtgggctttagtgactgccatatcaagttattggagcggctgcgcgtgaaagcgaaattagtagtgccaatacttcaagattcttatacgtggggcttactgatagcgcatcagtgcaattcgacaagagagtggcagctttttgaggttgagttgctgaaccaactggctacccaggttgcgatagcaattcaacagggctatttattctcgcaatgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z