BBa_K1916020
1
BBa_K1916020
CBCR9 + intein-CBD tag
2016-10-11T11:00:00Z
2016-10-12T12:13:54Z
Metagenomic data mining (JGI)
Fusion protein consisting of the GAF domain of an uncharacterized cyanobacteriochrome (CBCR) sequence and an intein/chitin-binding domain attached to the C-terminus for purification. Labelled as CBCR9.
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2493412
1
CBCR9
range2493412
1
1
516
annotation2493416
1
intein/chitin binding domain
range2493416
1
517
1299
BBa_K1916020_sequence
1
atggaacaacagcgagtcttatttgaagtagttgctaaaatccgggaatcacttgatctagatgctatttttcagacaactactcaagaaatctgtaaatcactgcaagcggatcgagttgcagtctatcgcttcgaccctgattggagtggtaaatatattgctgagtttgtaggtgatgggtgggtcaagttagtaagttctgatatcaagactgattggaaagatagctatttacaagaaacccaaggtgggcgatatcgccacaatgaaacctttgcagttgatgacatctataaggctggccactctgagtgtcatgttgcggctctggagcaatttcagacaaaggcgtatgcgatcgctccaatttttattgggcaacagctatggggcttattggcagcttaccaaaattctgcgccccgtcactgggaagcctcggaagttaagttcatcgcccagattgctaatcagctgggggttgcactccaacaaactgaattacttaaccagtgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z