BBa_K1916109 1 BBa_K1916109 Ara-Inducible Osc7112_5903g3 Expression System 2016-10-11T11:00:00Z 2016-10-12T11:22:44Z Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034. The Osc7112_5903g3 protein (see BBa_K1916009) under control of a strong pBAD promoter. RFC10 compatible. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2496081 1 osc7112_5903g3 range2496081 1 157 798 annotation2496078 1 BBa_K206000 range2496078 1 1 130 annotation2496083 1 BBa_K1916009 range2496083 1 157 1581 annotation2496080 1 BBa_B0034 range2496080 1 139 150 annotation2496082 1 intein/chitin binding domain range2496082 1 799 1581 annotation2496079 1 conserved range2496079 1 143 146 BBa_K1916109_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctatagagaaagaggagaaatactagatggaacaatcagaactggctatccgcaagcaaacagaaaaggcgatcgctttacaactgcaacggcagcgaactttgggagaaatcgcccagcaaattcgcgaatcgttggatattaacgaaattttggcgactgtgacacagcaagtcaaagaaatcttgcaaggcgatcggatcgtggtgttccggctatttggcgatggcagaagtcaaattgttgaagaagctgtgtctagcgagttccccgcgctcaaagaccatcactgggaggacgaactgtggtcgccagaaatcctcaaccggtactggcagggcaagccccgcatcgtccccgatgtcatgaccgatatctggactgattgcttggtggaatacgcaaccgtttgtcaagtccagtccaaaatcgttgcacctatcctgcaagaagtccgaagcagcgaaagccatagatgggttgccccagggcaaaccaagaagctctggggggttttagtagtccacgcctgtcgagaacaacgagtatggcaagagtcggaagcgcaactcctgcaacaaattgccaaccagttggcgatcgcaattcaacaagctagcctatttaagcagttgcagcaagaactcaccgaacggcccggggatccgtgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z