BBa_K1916110 1 BBa_K1916110 Ara-Inducible CBCR2 Expression System 2016-10-11T11:00:00Z 2016-10-12T11:42:29Z Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034. The CBCR2 protein (see BBa_K1916010) under control of a strong pBAD promoter. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2496124 1 CBCR2 range2496124 1 157 669 annotation2496118 1 promoter range2496118 1 1 131 annotation2496120 1 AraI2 range2496120 1 61 78 annotation2496122 1 conserved range2496122 1 143 146 annotation2496125 1 intein/chitin binding domain range2496125 1 670 1452 annotation2496119 1 AraI1 range2496119 1 40 57 annotation2496123 1 BBa_B0034 range2496123 1 139 150 annotation2496126 1 BBa_K1916010 range2496126 1 157 1452 annotation2496121 1 BBa_K206000 range2496121 1 1 130 BBa_K1916110_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctctagagaaagaggagaaatactagatggagcaagagcgactgctgcggctcattgtccaaaatattcatcaatcgctcaatctagacgaaatcctggcgacgatgctagacgaaacacggcagacgctccaggttgatcgggtaacggtttaccagtttgaacccgactggagtggtcgatttgtcgccgagtcggtcggggaagaatggatgcctctggtcgcggagggtgtgcagacggtttgggaagatacctatttgcaagacacccaagacgggcgctatcgccagaacgaaacctttgccgtcagcgatatttatcaggccgggcataccgactgccacattgaaattctggagcaatttcaggtgcgggcctatgcgatcgcccccatttttctggacgaacagctttgggggttactggcgatttatcaaaacacgggcccacgagactggcaagcctgggaactcgaactgctgcaacagatcagcctgcaaacggcgatcgccctgcgtcaatccaacctgtaccaatgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z