BBa_K1916111
1
BBa_K1916111
Ara-Inducible CBCR2 Expression System
2016-10-11T11:00:00Z
2016-10-12T11:47:04Z
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.
The CBCR2 protein (see BBa_K1916011) under control of a strong pBAD promoter. RFC10 compatible.
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2496103
1
BBa_K206000
range2496103
1
1
130
annotation2496108
1
BBa_K1916011
range2496108
1
157
1452
annotation2496106
1
CBCR2
range2496106
1
157
669
annotation2496100
1
promoter
range2496100
1
1
131
annotation2496105
1
BBa_B0034
range2496105
1
139
150
annotation2496102
1
AraI2
range2496102
1
61
78
annotation2496101
1
AraI1
range2496101
1
40
57
annotation2496104
1
conserved
range2496104
1
143
146
annotation2496107
1
intein/chitin binding domain
range2496107
1
670
1452
BBa_K1916111_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctatagagaaagaggagaaatactagatggagcaagagcgactgctgcggctcattgtccaaaatattcatcaatcgctcaacctagacgaaatcctggcgacgatgctagacgaaacacggcagacgctccaggttgatcgggtaacggtttaccagtttgaacccgactggagtggtcgatttgtcgccgagtcggtcggggaagaatggatgcctctggtcgcggagggtgtgcagacggtttgggaagatacctatttgcaagacacccaagacgggcgctatcgccagaacgaaacctttgccgtcagcgatatttatcaggccgggcataccgactgccacattgaaattctggagcaatttcaggtgcgggcctatgcgatcgcccccatttttctggacgaacagctttgggggttactggcgatttatcaaaacacgggcccacgagactggcaagcctgggaactcgaactgctgcaacagatcagcctgcaaacggcgatcgccctgcgtcaatccaacctgtaccaatgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z