BBa_K1916112
1
BBa_K1916112
Ara-Inducible CBCR3 Expression System
2016-10-12T11:00:00Z
2016-10-13T01:03:21Z
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.
The CBCR3 protein (see BBa_K1916012) under control of a strong pBAD promoter.
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2496217
1
intein/chitin binding domain
range2496217
1
679
1461
annotation2496218
1
BBa_K1916012
range2496218
1
157
1461
annotation2496216
1
CBCR3
range2496216
1
157
678
annotation2496211
1
AraI1
range2496211
1
40
57
annotation2496213
1
BBa_K206000
range2496213
1
1
130
annotation2496210
1
promoter
range2496210
1
1
131
annotation2496214
1
conserved
range2496214
1
143
146
annotation2496212
1
AraI2
range2496212
1
61
78
annotation2496215
1
BBa_B0034
range2496215
1
139
150
BBa_K1916112_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctctagagaaagaggagaaatactagatggagcaacagcagtccttgctagaagtggtcaccaaaattcgatcatcgctgacacctgaaatcatcttccgcacaaccacccaggaagggtgtcatctgctgaaggtagaacgggttgctgtctaccgttttgatgccgattggggaggtgcctttgttcaggactttgaatccaccacatccgagtggcagggtattctcaaattgggcgagaacacggtctgggatgacacctatttgcaagtcactcagggaggccgctatcgcaataatgaaacctttgctgtgaatgatatcgaaacggcgggcttgtactcctgtcatgttgatatattgaagcaatttcggattcgggcatttgcgatcgctccgatctttgtggggcaaaccctctgggggctgctggccgcctatcaacattcaagtccccgtaagtgggaaccttcggaagttcagtttctggctcagacagcagcgcaactcggcatggcactccagcaagcagaactgctagcccaatgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z