BBa_K1916112 1 BBa_K1916112 Ara-Inducible CBCR3 Expression System 2016-10-12T11:00:00Z 2016-10-13T01:03:21Z Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034. The CBCR3 protein (see BBa_K1916012) under control of a strong pBAD promoter. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2496214 1 conserved range2496214 1 143 146 annotation2496212 1 AraI2 range2496212 1 61 78 annotation2496218 1 BBa_K1916012 range2496218 1 157 1461 annotation2496217 1 intein/chitin binding domain range2496217 1 679 1461 annotation2496211 1 AraI1 range2496211 1 40 57 annotation2496215 1 BBa_B0034 range2496215 1 139 150 annotation2496213 1 BBa_K206000 range2496213 1 1 130 annotation2496210 1 promoter range2496210 1 1 131 annotation2496216 1 CBCR3 range2496216 1 157 678 BBa_K1916112_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctctagagaaagaggagaaatactagatggagcaacagcagtccttgctagaagtggtcaccaaaattcgatcatcgctgacacctgaaatcatcttccgcacaaccacccaggaagggtgtcatctgctgaaggtagaacgggttgctgtctaccgttttgatgccgattggggaggtgcctttgttcaggactttgaatccaccacatccgagtggcagggtattctcaaattgggcgagaacacggtctgggatgacacctatttgcaagtcactcagggaggccgctatcgcaataatgaaacctttgctgtgaatgatatcgaaacggcgggcttgtactcctgtcatgttgatatattgaagcaatttcggattcgggcatttgcgatcgctccgatctttgtggggcaaaccctctgggggctgctggccgcctatcaacattcaagtccccgtaagtgggaaccttcggaagttcagtttctggctcagacagcagcgcaactcggcatggcactccagcaagcagaactgctagcccaatgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z