BBa_K1916113 1 BBa_K1916113 Ara-Inducible CBCR3 Expression System 2016-10-12T11:00:00Z 2016-10-13T01:03:09Z Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034. The CBCR3 protein (see BBa_K1916013) under control of a strong pBAD promoter. RFC10 compatible. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2496224 1 BBa_B0034 range2496224 1 139 150 annotation2496221 1 AraI2 range2496221 1 61 78 annotation2496226 1 intein/chitin binding domain range2496226 1 679 1461 annotation2496227 1 BBa_K1916013 range2496227 1 157 1461 annotation2496225 1 CBCR3 range2496225 1 157 678 annotation2496222 1 BBa_K206000 range2496222 1 1 130 annotation2496220 1 AraI1 range2496220 1 40 57 annotation2496223 1 conserved range2496223 1 143 146 annotation2496219 1 promoter range2496219 1 1 131 BBa_K1916113_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctatagagaaagaggagaaatactagatggagcaacagcagtccttgctagaagtggtcaccaaaattcgatcatcgctgacacctgaaatcatcttccgcacaaccacccaggaagggtgtcatctgctgaaggtagaacgggttgctgtctaccgttttgatgccgattggggaggtgcctttgttcaggactttgaatccaccacatccgagtggcagggtattctcaaattgggcgagaacacggtctgggatgacacctatttgcaagtcactcagggaggccgctatcgcaataatgaaacctttgctgtgaatgatatcgaaacggcgggcttgtactcctgtcatgttgatatattgaagcaatttcggattcgggcatttgcgatcgctccgatctttgtggggcaaaccctctgggggctgctggccgcctatcaacattcaagtccccgtaagtgggaaccttcggaagttcagtttctggctcagacagcagcgcaactcggcatggcactccagcaagcagaactgctagcccaatgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z