BBa_K1916117 1 BBa_K1916117 Ara-Inducible CBCR6 Expression System 2016-10-12T11:00:00Z 2016-10-13T01:01:56Z Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034. The CBCR6 protein (see BBa_K1916017) under control of a strong pBAD promoter. RFC10 compatible. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2496255 1 promoter range2496255 1 1 131 annotation2496257 1 AraI2 range2496257 1 61 78 annotation2496256 1 AraI1 range2496256 1 40 57 annotation2496261 1 CBCR6 range2496261 1 157 675 annotation2496258 1 BBa_K206000 range2496258 1 1 130 annotation2496262 1 intein/chitin binding domain range2496262 1 676 1458 annotation2496259 1 conserved range2496259 1 143 146 annotation2496260 1 BBa_B0034 range2496260 1 139 150 annotation2496263 1 BBa_K1916017 range2496263 1 157 1458 BBa_K1916117_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctatagagaaagaggagaaatactagatggtcggtcaagtcgcggcgaaaattctcaagaacatcttgcgatcgctgaatattccccaagtcttgagtaacgccacacgggaaatccgtcagcaactggagtgcgatcgcgtagcggtgtatcggtttaatcaagattggagtggggaatttatcgccgaatccgttgctcctggctgggtacccttagtcggtccggatatcaaaacagtttgggaggatacctatctccaagaaacccgaggtgggcgttatcgtaacgaagaaaccaatggtgtgaatgacatttacgccgctgggttagctgattgtcacatcgatctgttggaacaatttgaagcaaaagcttacgcgatcgcgcccatttttgaaggagaaaaactctggggattactcgccgccttccaaaactcaggtccgcgtcaatgggaagcctcagagcttaacatactggcattagtcgggcgtcagtttggtgtcgccctccagcaagccgaatacctggacagtttacaatgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z