BBa_K1916118 1 BBa_K1916118 Ara-Inducible CBCR8 Expression System 2016-10-12T11:00:00Z 2016-10-13T12:53:43Z Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034. The CBCR8 protein (see BBa_K1916018) under control of a strong pBAD promoter. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2496349 1 promoter range2496349 1 1 131 annotation2496354 1 BBa_B0034 range2496354 1 139 150 annotation2496357 1 BBa_K1916018 range2496357 1 157 1509 annotation2496350 1 AraI1 range2496350 1 40 57 annotation2496355 1 CBCR8 range2496355 1 157 726 annotation2496352 1 BBa_K206000 range2496352 1 1 130 annotation2496351 1 AraI2 range2496351 1 61 78 annotation2496353 1 conserved range2496353 1 143 146 annotation2496356 1 intein/chitin binding domain range2496356 1 727 1509 BBa_K1916118_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctctagagaaagaggagaaatactagatggaacgggaaagagcgatcacgcaagtgattcaaaggatgcgccaaactctggatctggatactatatttactgcaacaacccaagaattgcggcaagtacttaactgcgatcgggtcgttgtctatcgtttcaatccccactggcgtggtgaatttgtttctgagtcggtggggaatggttggacttctctaatagaagaatacaacaatgaccccaatctcacagaagatgttttagaaaatggcctttgcctagcgaaaatattgcatggtgcggatacccaagagcaagacgctgatttgcaaacaacccaagatgcaagttttagctgtgtcccagacatttacacagctgggtttaatccttgttatatcaaactcttggagcgttttcaggcaaaagcttatattattgtccccatcttttgtggtagtcaactttggggattattggcgagttatcaaaattctggttcccgtcaatggaaaccagaagagatcaacattggagttcaaattggcaatcaattgggagttgctttgcagcaagcacaattactcgcacaatgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z