BBa_K1916119
1
BBa_K1916119
Ara-Inducible CBCR8 Expression System
2016-10-12T11:00:00Z
2016-10-13T12:54:40Z
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.
The CBCR8 protein (see BBa_K1916019) under control of a strong pBAD promoter. RFC10 compatible.
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2496364
1
CBCR8
range2496364
1
157
726
annotation2496360
1
AraI2
range2496360
1
61
78
annotation2496361
1
BBa_K206000
range2496361
1
1
130
annotation2496365
1
intein/chitin binding domain
range2496365
1
727
1509
annotation2496363
1
BBa_B0034
range2496363
1
139
150
annotation2496366
1
BBa_K1916019
range2496366
1
157
1509
annotation2496358
1
promoter
range2496358
1
1
131
annotation2496362
1
conserved
range2496362
1
143
146
annotation2496359
1
AraI1
range2496359
1
40
57
BBa_K1916119_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctatagagaaagaggagaaatactagatggaacgggaaagagcgatcacgcaagtgattcaaaggatgcgccaaactctggatctggatactatatttactgcaacaacccaagaattgcggcaagtacttaactgcgatcgggtcgttgtctatcgtttcaatccccactggcgtggtgaatttgtttctgagtcggtggggaatggttggacttctctaatagaagaatacaacaatgaccccaatctcacagaagatgttttagaaaatggcctttgcctagcgaaaatattgcatggtgcggatacccaagagcaagacgctgatttgcaaacaacccaagatgcaagttttagctgtgtcccagacatttacacagctgggtttaatccttgttatatcaaactcttggagcgttttcaggcaaaagcttatattattgtccccatcttttgtggtagtcaactttggggattattggcgagttatcaaaattctggttcccgtcaatggaaaccagaagagatcaacattggagttcaaattggcaatcaattgggagttgctttgcagcaagcacaattactcgcacaatgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z