BBa_K1916119 1 BBa_K1916119 Ara-Inducible CBCR8 Expression System 2016-10-12T11:00:00Z 2016-10-13T12:54:40Z Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034. The CBCR8 protein (see BBa_K1916019) under control of a strong pBAD promoter. RFC10 compatible. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2496361 1 BBa_K206000 range2496361 1 1 130 annotation2496359 1 AraI1 range2496359 1 40 57 annotation2496366 1 BBa_K1916019 range2496366 1 157 1509 annotation2496360 1 AraI2 range2496360 1 61 78 annotation2496358 1 promoter range2496358 1 1 131 annotation2496364 1 CBCR8 range2496364 1 157 726 annotation2496362 1 conserved range2496362 1 143 146 annotation2496363 1 BBa_B0034 range2496363 1 139 150 annotation2496365 1 intein/chitin binding domain range2496365 1 727 1509 BBa_K1916119_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctatagagaaagaggagaaatactagatggaacgggaaagagcgatcacgcaagtgattcaaaggatgcgccaaactctggatctggatactatatttactgcaacaacccaagaattgcggcaagtacttaactgcgatcgggtcgttgtctatcgtttcaatccccactggcgtggtgaatttgtttctgagtcggtggggaatggttggacttctctaatagaagaatacaacaatgaccccaatctcacagaagatgttttagaaaatggcctttgcctagcgaaaatattgcatggtgcggatacccaagagcaagacgctgatttgcaaacaacccaagatgcaagttttagctgtgtcccagacatttacacagctgggtttaatccttgttatatcaaactcttggagcgttttcaggcaaaagcttatattattgtccccatcttttgtggtagtcaactttggggattattggcgagttatcaaaattctggttcccgtcaatggaaaccagaagagatcaacattggagttcaaattggcaatcaattgggagttgctttgcagcaagcacaattactcgcacaatgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z