BBa_K1916120
1
BBa_K1916120
Ara-Inducible CBCR9 Expression System
2016-10-12T11:00:00Z
2016-10-13T12:53:50Z
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.
The CBCR9 protein (see BBa_K1916020) under control of a strong pBAD promoter.
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2496374
1
intein/chitin binding domain
range2496374
1
673
1455
annotation2496373
1
CBCR9
range2496373
1
157
672
annotation2496372
1
BBa_B0034
range2496372
1
139
150
annotation2496375
1
BBa_K1916020
range2496375
1
157
1455
annotation2496369
1
AraI2
range2496369
1
61
78
annotation2496367
1
promoter
range2496367
1
1
131
annotation2496368
1
AraI1
range2496368
1
40
57
annotation2496370
1
BBa_K206000
range2496370
1
1
130
annotation2496371
1
conserved
range2496371
1
143
146
BBa_K1916120_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctctagagaaagaggagaaatactagatggaacaacagcgagtcttatttgaagtagttgctaaaatccgggaatcacttgatctagatgctatttttcagacaactactcaagaaatctgtaaatcactgcaagcggatcgagttgcagtctatcgcttcgaccctgattggagtggtaaatatattgctgagtttgtaggtgatgggtgggtcaagttagtaagttctgatatcaagactgattggaaagatagctatttacaagaaacccaaggtgggcgatatcgccacaatgaaacctttgcagttgatgacatctataaggctggccactctgagtgtcatgttgcggctctggagcaatttcagacaaaggcgtatgcgatcgctccaatttttattgggcaacagctatggggcttattggcagcttaccaaaattctgcgccccgtcactgggaagcctcggaagttaagttcatcgcccagattgctaatcagctgggggttgcactccaacaaactgaattacttaaccagtgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z