BBa_K1916121 1 BBa_K1916121 Ara-Inducible CBCR9 Expression System 2016-10-12T11:00:00Z 2016-10-13T12:54:47Z Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034. The CBCR9 protein (see BBa_K1916021) under control of a strong pBAD promoter. RFC10 compatible. false false _2383_ 31626 31626 9 false It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns. false Jonathan Chen annotation2496382 1 CBCR9 range2496382 1 157 672 annotation2496384 1 BBa_K1916021 range2496384 1 157 1455 annotation2496376 1 promoter range2496376 1 1 131 annotation2496383 1 intein/chitin binding domain range2496383 1 673 1455 annotation2496377 1 AraI1 range2496377 1 40 57 annotation2496380 1 conserved range2496380 1 143 146 annotation2496381 1 BBa_B0034 range2496381 1 139 150 annotation2496378 1 AraI2 range2496378 1 61 78 annotation2496379 1 BBa_K206000 range2496379 1 1 130 BBa_K1916121_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctatagagaaagaggagaaatactagatggaacaacagcgagtcttatttgaagtagttgctaaaatccgggaatcacttgatctggatgctatttttcagacaactactcaagaaatctgtaaatcactgcaagcggatcgagttgcagtctatcgcttcgaccctgattggagtggtaaatatattgctgagtttgtaggtgatgggtgggtcaagttagtaagttctgatatcaagactgattggaaagatagctatttacaagaaacccaaggtgggcgatatcgccacaatgaaacctttgcagttgatgacatctataaggctggccactctgagtgtcatgttgcggctctggagcaatttcagacaaaggcgtatgcgatcgctccaatttttattgggcaacagctatggggcttattggcagcttaccaaaattctgcgccccgtcactgggaagcctcggaagttaagttcatcgcccagattgctaatcagctgggggttgcactccaacaaactgaattacttaaccagtgcatcacgggagatgccctagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z