BBa_K1916122
1
BBa_K1916122
Ara-Inducible CBCR11 Expression System
2016-10-12T11:00:00Z
2016-10-13T12:54:06Z
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.
The CBCR11 protein (see BBa_K1916022) under control of a strong pBAD promoter. RFC10 compatible.
false
false
_2383_
31626
31626
9
false
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
false
Jonathan Chen
annotation2496386
1
AraI1
range2496386
1
40
57
annotation2496385
1
promoter
range2496385
1
1
131
annotation2496392
1
intein/chitin binding domain
range2496392
1
670
1452
annotation2496387
1
AraI2
range2496387
1
61
78
annotation2496388
1
BBa_K206000
range2496388
1
1
130
annotation2496393
1
BBa_K1916022
range2496393
1
157
1452
annotation2496389
1
conserved
range2496389
1
143
146
annotation2496391
1
CBCR11
range2496391
1
157
669
annotation2496390
1
BBa_B0034
range2496390
1
139
150
BBa_K1916122_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctctagagaaagaggagaaatactagatgacccaggaaacggtatccagcgttctgactaagttgagctatggcgaggatatcgccaccatctacaacacatccactcgtgaactgcggaaactactcaagtgcgatcgcacggcggtgtaccagtttgaaccggactggagtggcgtgtttgtcgccgagtcggtggcgacgggatggacacccttagttggtccggatatccgcaccgtctgggaagacacctatttacaagaaactcaagggggtcgttatcggaatcgggaaagctttgccgtggatgatatccataccgtgggtcactccgattgtcacgttgaaattttagaacagtttgaggtcaaagcttacgccctcgccccgattttcgtggctgatgagttatggggaatcctctgtgcttatcaaaacactggatctcgccattggacgagtaccgaaatcactgcattgacccaacttgctcaaggggtgggaacggcattacaacgcctatactatctagaacagtgcatcacgggagatgcactagttgccctacccgagggcgagtcggtacgcatcgccgacatcgtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcgaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcgggaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgcgacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcacgaacgggttcgtcagccacgctactggcctcaccggtctgaactcaggcctcacgacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z