LOCUS       BBa_K1919003            2470 bp    DNA     linear       28-Jun-2024
DEFINITION  T7 promotor (under the control of lacI) and downstream CecropinXJ
ACCESSION   BBa_K1919003
VERSION     BBa_K1919003.1  
FEATURES             Location/Qualifiers
     promoter        1084..1161
                     /label=lacI promotor
     promoter        1474..1492
                     /label=T7 promotor
     misc_feature    1493..1517
                     /label=lac operator
     misc_feature    1562..1888
                     /label=TrxA
     misc_feature    1910..1927
                     /label=6xHis Tag
     misc_feature    1937..1954
                     /label=Thrombin site
     misc_feature    1961..2005
                     /label=S tag
     misc_feature    2021..2035
                     /label=enterokinase site
     CDS             2057..2245
                     /label=cecropinXJ
     misc_feature    2342..2389
                     /label=T7 terminator
     misc_feature    2399..2444
                     /label=rrnB T1 terminator
     CDS             1..1083
                     /label=lacI
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     1201 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     1261 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     1321 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     1381 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     1441 agaggatcga gatcgatctc gatcccgcga aattaatacg actcactata ggggaattgt
     1501 gagcggataa caattcccct caagaaataa ttttgtttaa ctttaagaag gagatataca
     1561 tatgagcgat aaaattattc acctgactga cgacagtttt gacacggatg tactcaaagc
     1621 ggacggggcg atcctcgtcg atttctgggc agagtggtgc ggtccgtgca aaatgatcgc
     1681 cccgattctg gatgaaatcg ctgacgaata tcagggcaaa ctgaccgttg caaaactgaa
     1741 catcgatcaa aaccctggca ctgcgccgaa atatggcatc cgtggtatcc cgactctgct
     1801 gctgttcaaa aacggtgaag tggcggcaac caaagtgggt gcactgtcta aaggtcagtt
     1861 gaaagagttc ctcgacgcta acctggccgg ttctggttct ggccatatgc accatcatca
     1921 tcatcattct tctggtctgg tgccacgcgg ttctggtatg aaagaaaccg ctgctgctaa
     1981 attcgaacgc cagcacatgg acagcccaga tctgggtacc gacgacgacg acaaggccat
     2041 ggctgatatc ggatccatga acttcgctaa aatcctgtct ttcgttttcg ctctggttct
     2101 ggctctgtct atgacctctg ctgctccgga accgcgttgg aaaatcttca aaaaaatcga
     2161 aaaaatgggt cgtaacatcc gtgacggtat cgttaaagct ggtccggcta tcgaagttct
     2221 gggttctgct aaagctatcg gtaaataata actcgagcac caccaccacc accactgaga
     2281 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     2341 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc ccaggcatca
     2401 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2461 gaacgctctc
//