BBa_K1919200 1 BBa_K1919200 J23100 promoter-Constitutive AarC Expression Device 2016-10-13T11:00:00Z 2016-10-19T12:15:14Z The coding sequence of AarC comes from BBa_K1919233. This device can express AarC with His-tag. Promoter is BBa_J23100. His-tag and T7 terminator come from pET-23 vector. false false _2386_ 26170 26170 9 false The AarC conding sequence with His-tag and T7 terminator were cloned from pET-23-AarC express vector. Promoter and RBS were added by PCR. false Haoyu Wang annotation2520520 1 T7 terminator range2520520 1 1669 1714 annotation2520500 1 His tag range2520500 1 1584 1601 annotation2520495 1 AarC coding sequence range2520495 1 63 1577 annotation2520541 1 ATG codon range2520541 1 63 65 annotation2520502 1 single TAA stop codon range2520502 1 1616 1618 annotation2501410 1 BBa_J23100 range2501410 1 1 35 annotation2520493 1 BBa_B0032 range2520493 1 44 56 BBa_K1919200_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgaccgaacgtatccgtaacgtggcgctgcgtagcaaggtttgcccggcggagaccgcgagcgaactgattaaacacggtgacgtggttggtaccagcggtttcaccggtgcgggttacccgaaggaagtgccgaaagcgctggcgcagcgtatggaagcggcgcacgaccgtggcgagaagtatcagatcagcctgattaccggtgcgagcaccggtccgcaactggatggcgagctggcgaaagcgaacggtgtttacttccgtagcccgtttaacaccgacgcgaccatgcgtaaccgtatcaacgcgggcgagaccgaatacttcgataaccacctgggtcaggtggcgggtcgtgcggttcagggtaactatggcaagtttaacattgcgctggttgaggcgaccgcgatcaccgaggacggtggcattgtgccgaccagcagcgttggtaacagccagaccttcctgaacctggcggaaaaagtgatcattgaggttaacgaatggcaaaacccgatgctggagggcatccacgacatttgggatggtaacgtgagcggcgtttcgacccgtgacatcgtgccgattgttcgtgcggatcagcgtgtgggtggcccggtgctgcgtgttaacccggataagatcgcgtcgattgttcgtaccaacgaccgtgatcgtaacgcgccgtttgcggcgccggacgaaaccgcgaaggcgatcgcgggttacctgctggatttctttggtcacgaggtgaaacagaaccgtctgccgccgagcctgctgccgctgcaaagcggtgtgggcaacgttgcgaacgcggttctggagggtctgaaagaaggcccgttcgagaacctggtgggctatagcgaagttatccaagacggtatgctggcgatgctggatagcggccgtatgcgtattgcgagcgcgagcagctttagcctgagcccggaggcggcggaggaaatcaacaaccgtatggacttctttcgtagcaagatcattctgcgtcagcaagatgttagcaacagcccgggtatcattcgtcgtctgggctgcatcgcgatgaacggtatgatcgaagcggacatttacggtaacgtgaacagcacccgtgttatgggcagcaaaatgatgaacggtatcggtggcagcggcgatttcgcgcgtagcagctatctgagcatttttctgagcccgagcaccgcgaagggtggcaaaatcagcgcgattgtgccgatggcggcgcacgttgaccacatcatgcaggatgcgcaaattttcgtgaccgaacagggtctggcggacctgcgtggtctgagcccggttcaacgtgcgcgtgagatcattagcaagtgcgcgcacccggattaccgtccgatgctgcaagactatttcgatcgtgcgctgaagaacagctttggtaaacacaccccgcacctgctgaccgaggcgctgagctggcaccaacgttttatcgacaccggcaccatgctgccgagcctcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggtttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z