BBa_K1919204 1 BBa_K1919204 J23104 promoter-Constitutive AarC Expression Device 2016-10-13T11:00:00Z 2016-10-19T01:07:37Z The coding sequence of AarC comes from BBa_K1919233. This device can express AarC with His-tag. Promoter is BBa_J23104. His-tag and T7 terminator come from pET-23 vector. false false _2386_ 26170 26170 9 false The AarC conding sequence with His-tag and T7 terminator were cloned from pET-23-AarC express vector. Promoter and RBS were added by PCR. false Haoyu Wang annotation2520543 1 BBa_B0032 range2520543 1 44 56 annotation2520544 1 ATG start codon range2520544 1 63 65 annotation2520547 1 His tag range2520547 1 1584 1601 annotation2520542 1 BBa_J23104 range2520542 1 1 35 annotation2520545 1 AarC coding sequence range2520545 1 63 1577 annotation2520546 1 single TAA stop codon range2520546 1 1616 1618 annotation2520548 1 T7 terminator range2520548 1 1669 1714 BBa_K1919204_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatgaccgaacgtatccgtaacgtggcgctgcgtagcaaggtttgcccggcggagaccgcgagcgaactgattaaacacggtgacgtggttggtaccagcggtttcaccggtgcgggttacccgaaggaagtgccgaaagcgctggcgcagcgtatggaagcggcgcacgaccgtggcgagaagtatcagatcagcctgattaccggtgcgagcaccggtccgcaactggatggcgagctggcgaaagcgaacggtgtttacttccgtagcccgtttaacaccgacgcgaccatgcgtaaccgtatcaacgcgggcgagaccgaatacttcgataaccacctgggtcaggtggcgggtcgtgcggttcagggtaactatggcaagtttaacattgcgctggttgaggcgaccgcgatcaccgaggacggtggcattgtgccgaccagcagcgttggtaacagccagaccttcctgaacctggcggaaaaagtgatcattgaggttaacgaatggcaaaacccgatgctggagggcatccacgacatttgggatggtaacgtgagcggcgtttcgacccgtgacatcgtgccgattgttcgtgcggatcagcgtgtgggtggcccggtgctgcgtgttaacccggataagatcgcgtcgattgttcgtaccaacgaccgtgatcgtaacgcgccgtttgcggcgccggacgaaaccgcgaaggcgatcgcgggttacctgctggatttctttggtcacgaggtgaaacagaaccgtctgccgccgagcctgctgccgctgcaaagcggtgtgggcaacgttgcgaacgcggttctggagggtctgaaagaaggcccgttcgagaacctggtgggctatagcgaagttatccaagacggtatgctggcgatgctggatagcggccgtatgcgtattgcgagcgcgagcagctttagcctgagcccggaggcggcggaggaaatcaacaaccgtatggacttctttcgtagcaagatcattctgcgtcagcaagatgttagcaacagcccgggtatcattcgtcgtctgggctgcatcgcgatgaacggtatgatcgaagcggacatttacggtaacgtgaacagcacccgtgttatgggcagcaaaatgatgaacggtatcggtggcagcggcgatttcgcgcgtagcagctatctgagcatttttctgagcccgagcaccgcgaagggtggcaaaatcagcgcgattgtgccgatggcggcgcacgttgaccacatcatgcaggatgcgcaaattttcgtgaccgaacagggtctggcggacctgcgtggtctgagcccggttcaacgtgcgcgtgagatcattagcaagtgcgcgcacccggattaccgtccgatgctgcaagactatttcgatcgtgcgctgaagaacagctttggtaaacacaccccgcacctgctgaccgaggcgctgagctggcaccaacgttttatcgacaccggcaccatgctgccgagcctcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggtttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z