BBa_K1919301 1 BBa_K1919301 livF 2016-10-13T11:00:00Z 2016-10-21T12:07:22Z The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company. LivF encodes a subunit of leucine ABC tansporter. This device constructed in our team contains a promoter J23100, a RBS, livF coding region and a terminator. The device can be transferred into E.coli and overexpress livF directly. The expression of the livF RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been overexpressed in E.coli BL21 and E.coli DH5a. false false _2386_ 27483 27483 9 false The restriction sites are eliminated by mutation. false Shaobo Yang annotation2516410 1 B0030 range2516410 1 44 58 annotation2516412 1 B0010 range2516412 1 778 858 annotation2516409 1 J23100 range2516409 1 1 35 annotation2516411 1 livF coding region range2516411 1 65 777 BBa_K1919301_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaataaataatggaaaaagtcatgttgtcctttgacaaagtcagcgcccactacggcaaaatccaggcgctgcatgaggtgagcctgcatatcaatcagggcgagattgtcacgctgattggcgcgaacggggcggggaaaaccaccttgctcggcacgttatgcggcgatccgcgtgccaccagcgggcgaattgtgtttgatgataaagacattaccgactggcagacagcgaaaatcatgcgcgaagcggtggcgattgtcccggaagggcgtcgcgtcttctcgcggatgacggtggaagagaacctggcgatgggcggtttttttgctgaacgcgaccagttccaggagcgcataaagtgggtgtatgagctgtttccacgtctgcatgagcgccgtattcagcgggcgggcaccatgtccggcggtgaacagcagatgctggcgattggtcgtgcgctgatgagcaacccgcgtttgctactgcttgatgagccatcgctcggtcttgcgccgattatcatccagcaaattttcgacaccatcgagcagctgcgcgagcaggggatgactatctttctcgtcgagcagaacgccaaccaggcgctaaagctggcggatcgcggctacgtgctggaaaacggccatgtagtgctttccgatactggtgatgcgctgctggcgaatgaagcggtgagaagtgcgtatttaggcgggtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z