BBa_K1919302 1 BBa_K1919302 livG 2016-10-13T11:00:00Z 2016-10-21T12:09:25Z The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company. LivG is an ATP-binding cassette (ABC) protein that is a component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. The device encodes livG protein, one of a subunit of leucine ABC transporter. The LivG protein sequence contains Walker A and Walker B motifs involved in the binding and hydrolysis of ATP. false false _2386_ 27483 27483 9 false The restriction sites are eliminated by mutation. false Shaobo Yang annotation2516416 1 livG coding region range2516416 1 57 832 annotation2516417 1 B0010 range2516417 1 832 912 annotation2516414 1 J23100 range2516414 1 1 35 annotation2516415 1 B0030 range2516415 1 44 58 BBa_K1919302_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaataaataatgagtcagccattattatctgttaacggcctgatgatgcgcttcggcggcctgctggcggtgaacaacgtcaatcttgaactgtacccgcaggagatcgtctcgttaatcggccctaacggtgccggaaaaaccacggtttttaactgtctgaccggattctacaaacccaccggcggcaccattttactgcgcgatcagcacctggaaggtttaccggggcagcaaattgcccgcatgggcgtggtgcgcaccttccagcatgtgcgtctgttccgtgaaatgacggtaattgaaaacctgctggtggcgcagcatcagcaactgaaaaccgggctgttctctggcctgttgaaaacgccatccttccgtcgcgcccagagcgaagcgctcgaccgcgccgcgacctggcttgagcgcattggtttgctggaacacgccaaccgtcaggcgagtaacctggcctatggtgaccagcgccgtcttgagattgcccgctgcatggtgacgcagccggagattttaatgctcgacgaacctgcggcaggtcttaacccgaaagagacgaaagagctggatgagctgattgccgaactgcgcaatcatcacaacaccactatcttgttgattgaacacgatatgaagctggtgatgggaatttcggaccgaatttacgtggtcaatcaggggacgccgctggcaaacggtacgccggagcagatccgtaataacccggacgtgatccgtgcctatttaggtgaggcataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z