BBa_K1919303
1
BBa_K1919303
livH
2016-10-13T11:00:00Z
2016-10-21T12:11:10Z
The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company.
LivH is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. LivH encodes a very hydrophobic protein with 7 or 9 predicted transmembrane regions.
This device constructed in our team contains a promoter J23100, a RBS, livH coding region and a terminator. The device can be transferred into E.coli and overexpress livH directly. The expression of the livH RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livH has been highly expressed in E.coli BL21 and E.coli DH5a.
false
false
_2386_
27483
27483
9
false
The restriction sites are eliminated by mutation.
false
Shaobo Yang
annotation2516422
1
livH coding region
range2516422
1
65
992
annotation2516421
1
B0030
range2516421
1
44
58
annotation2516420
1
J23100
range2516420
1
1
35
annotation2516423
1
B0010
range2516423
1
993
1071
BBa_K1919303_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaataaataatgtctgagcagtttttgtatttcttgcagcagatgtttaacggcgtcacgctgggcagtacctacgcgctgatagccatcggctacaccatggtttacggcattatcggcatgatcaacttcgcccacggcgaggtttatatgattggcagctacgtctcatttatgatcatcgccgcgctgatgatgatgggcattgataccggctggctgctggtagctgcgggattcgtcggcgcaatcgtcattgccagcgcctacggctggagtatcgaacgggtggcttaccgcccggtgcgtaactctaagcgcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcctgaccgaaggttcgcgcgacgtggcgctgccgagcctgtttaacggtcagtgggtggtggggcatagcgaaaacttctctgcctctattaccaccatgcaggcggtgatctggattgttaccttcctcgccatgctggcgctgacgattttcattcgctattcccgcatgggtcgcgcgtgtcgtgcctgcgcggaagatctgaaaatggcgagtctgcttggcattaacaccgaccgggtgattgcgctgacctttgtgattggcgcggcgatggcggcggtggcgggtgtgctgctcggtcagttctacggcgtcattaacccctacatcggctttatggccgggatgaaagcctttaccgcggcggtgctcggtgggattggcagcattccgggagcgatgattggcggcctgattctggggattgcggaggcgctctcttctgcctatctgagtacggaatataaagatgtggtctcattcgccctgctgattctggtgctgctggtgatgccgaccggtattctgggtcgcccggaggtagagaaagtatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z