BBa_K1919303 1 BBa_K1919303 livH 2016-10-13T11:00:00Z 2016-10-21T12:11:10Z The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company. LivH is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. LivH encodes a very hydrophobic protein with 7 or 9 predicted transmembrane regions. This device constructed in our team contains a promoter J23100, a RBS, livH coding region and a terminator. The device can be transferred into E.coli and overexpress livH directly. The expression of the livH RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livH has been highly expressed in E.coli BL21 and E.coli DH5a. false false _2386_ 27483 27483 9 false The restriction sites are eliminated by mutation. false Shaobo Yang annotation2516420 1 J23100 range2516420 1 1 35 annotation2516421 1 B0030 range2516421 1 44 58 annotation2516422 1 livH coding region range2516422 1 65 992 annotation2516423 1 B0010 range2516423 1 993 1071 BBa_K1919303_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaataaataatgtctgagcagtttttgtatttcttgcagcagatgtttaacggcgtcacgctgggcagtacctacgcgctgatagccatcggctacaccatggtttacggcattatcggcatgatcaacttcgcccacggcgaggtttatatgattggcagctacgtctcatttatgatcatcgccgcgctgatgatgatgggcattgataccggctggctgctggtagctgcgggattcgtcggcgcaatcgtcattgccagcgcctacggctggagtatcgaacgggtggcttaccgcccggtgcgtaactctaagcgcctgattgcactcatctctgcaatcggtatgtccatcttcctgcaaaactacgtcagcctgaccgaaggttcgcgcgacgtggcgctgccgagcctgtttaacggtcagtgggtggtggggcatagcgaaaacttctctgcctctattaccaccatgcaggcggtgatctggattgttaccttcctcgccatgctggcgctgacgattttcattcgctattcccgcatgggtcgcgcgtgtcgtgcctgcgcggaagatctgaaaatggcgagtctgcttggcattaacaccgaccgggtgattgcgctgacctttgtgattggcgcggcgatggcggcggtggcgggtgtgctgctcggtcagttctacggcgtcattaacccctacatcggctttatggccgggatgaaagcctttaccgcggcggtgctcggtgggattggcagcattccgggagcgatgattggcggcctgattctggggattgcggaggcgctctcttctgcctatctgagtacggaatataaagatgtggtctcattcgccctgctgattctggtgctgctggtgatgccgaccggtattctgggtcgcccggaggtagagaaagtatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z