BBa_K1919304 1 BBa_K1919304 livK 2016-10-13T11:00:00Z 2016-10-21T12:12:25Z The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company. LivK is the periplasmic binding protein of the LS or leucine specific ABC transport system in E. coli K-12.LivK is synthesized as a precursor; a 23 amino acid signal sequence is cleaved upon export to the periplasm.The protein consists of two domains connected by a 3-stranded hinge; in the closed form, ligands (phenylalanine and leucine) are bound in a cleft formed between the two domains; binding is stabilized by hydrogen bonds and van der Waals contacts. This device constructed in our team contains a promoter J23100, a RBS, livK coding region and a terminator. The device can be transferred into E.coli and overexpress livK directly. The expression of the livK RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livK has been highly expressed in E.coli BL21 and E.coli DH5a. false false _2386_ 27483 27483 9 false The restriction sites are eliminated by mutation. false Shaobo Yang annotation2516428 1 B0010 range2516428 1 1176 1254 annotation2516426 1 B0030 range2516426 1 44 58 annotation2516427 1 livK coding region range2516427 1 65 1175 annotation2516425 1 J23100 range2516425 1 1 35 BBa_K1919304_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaataaataatgaaacggaatgcgaaaactatcatcgcagggatgattgcactggcaatttcacacaccgctatggctgacgatattaaagtcgccgttgtcggcgcgatgtccggcccgattgcccagtggggcgatatggaatttaacggcgcgcgtcaggcaattaaagacattaatgccaaagggggaattaagggcgataaactggttggcgtggaatatgacgacgcatgcgacccgaaacaagccgttgcggtcgccaacaaaatcgttaatgacggcattaaatacgttattggtcatctgtgttcttcttctacccagcctgcgtcagatatctatgaagacgaaggtattctgatgatctcgccgggagcgaccaacccggagctgacccaacgcggttatcaacacattatgcgtactgccgggctggactcttcccaggggccaacggcggcaaaatacattcttgagacggtgaagccccagcgcatcgccatcattcacgacaaacaacagtatggcgaagggctggcgcgttcggtgcaggacgggctgaaagcggctaacgccaacgtcgtcttcttcgacggtattaccgccggggagaaagatttctccgcgctgatcgcccgcctgaaaaaagaaaacatcgacttcgtttactacggcggttactacccggaaatggggcagatgctgcgccaggcccgttccgttggcctgaaaacccagtttatggggccggaaggtgtgggtaatgcgtcgttgtcgaacattgccggtgatgccgccgaaggcatgttggtcactatgccaaaacgctatgaccaggatccggcaaaccagggcatcgttgatgcgctgaaagcagacaagaaagatccgtccgggccttatgtctggatcacctacgcggcggtgcaatctctggcgactgcccttgagcgtaccggcagcgatgagccgctggcgctggtgaaagatttaaaagctaacggtgcaaacaccgtgattgggccgctgaactgggatgaaaaaggcgatcttaagggatttgattttggtgtcttccagtggcacgccgacggttcatccacggcagccaagtgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z