BBa_K1919305 1 BBa_K1919305 livM 2016-10-13T11:00:00Z 2016-10-21T12:14:01Z The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company. LivM is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. livM encodes a very hydrophobic protein with 9 predicted transmembrane regions. This device constructed in our team contains a promoter J23100, a RBS, livM coding region and a terminator. The device can be transferred into E.coli and overexpress livM directly. The expression of the livM RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livM has been highly expressed in E.coli BL21 and E.coli DH5a. false false _2386_ 27483 27483 9 false The restriction sites are eliminated by mutation. false Shaobo Yang annotation2516431 1 livM coding region range2516431 1 65 1343 annotation2516429 1 J23100 range2516429 1 1 35 annotation2516432 1 B0010 range2516432 1 1344 1422 annotation2516430 1 B0030 range2516430 1 44 58 BBa_K1919305_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaataaataatgaaaccgatgcatattgcaatggcgctgctctctgccgcgatgttctttgtgctggcgggcgtctttatgggcgtgcaactggagctggatggcaccaaactggtggtcgacacggcttcggatgtccgttggcagtgggtgtttatcggcacggcggtggtctttttcttccagcttttgcgaccggctttccagaaagggttgaaaagcgtttccggaccgaagtttattctgcccgccattgatggctccacggtgaagcagaaactgttcctcgtggcgctgttggtgcttgcggtggcgtggccgtttatggtttcacgcgggacggtggatattgccaccctgaccatgatctacattatcctcggtctcgggctgaacgtggttgttggtctttctggtctgctggtgctggggtacggcggtttttacgccatcggcgcttacacttttgcgctgctcaatcactattacggcttgggcttctggacctgcctgccgattgctggattaatggcagcggcggcgggcttcctgctcggttttccggtgctgcgtttgcgcggtgactatctggcgatcgttaccctcggtttcggcgaaattgtgcgcatattgctgctcaataacaccgaaattaccggcggcccgaacggaatcagtcagatcccgaaaccgacactcttcggactcgagttcagccgtaccgctcgtgaaggcggctgggacacgttcagtaatttctttggcctgaaatacgatccctccgatcgtgtcatcttcctctacctggtggcgttgctgctggtggtgctaagcctgtttgtcattaaccgcctgctgcggatgccgctggggcgtgcgtgggaagcgttgcgtgaagatgaaatcgcctgccgttcgctgggcttaagcccgcgtcgtatcaagctgactgcctttaccataagtgccgcgtttgccggttttgccggaacgctgtttgcggcgcgtcagggctttgtcagcccggaatccttcacctttgccgaatcggcgtttgtgctggcgatagtggtgctcggcggtatgggctcgcaatttgcggtgattctggcggcaattttgctggtggtgtcgcgcgagttgatgcgtgatttcaacgaatacagcatgttaatgctcggtggtttgatggtgctgatgatgatctggcgtccgcagggcttgctgcccatgacgcgcccgcaactgaagctgaaaaacggcgcagcgaaaggagagcaggcatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z