LOCUS       BBa_K1919500            1581 bp    DNA     linear       25-Nov-2024
DEFINITION  K1321200+J04450 (Vitreoscilla hemoglobin + mRFP)
ACCESSION   BBa_K1919500
VERSION     BBa_K1919500.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23101 constitutive
     misc_feature    1..504
                     /label=BBa_K1321200
     RBS             44..55
                     /label=B0034
     misc_feature    64..66
                     /label=Start
     CDS             64..504
                     /label=VHb
     misc_feature    502..504
                     /label=Stop
     misc_feature    513..712
                     /label=BBa_R0010
     misc_feature    513..1581
                     /label=BBa_J04450
     misc_feature    601..638
                     /label=CAP
     promoter        649..679
                     /label=lac promoter
     misc_feature    721..732
                     /label=BBa_B0034
     CDS             739..1413
                     /label=mrfp1
     misc_feature    739..1419
                     /label=BBa_E1010
     misc_feature    1453..1532
                     /label=BBa_B0010
     stem_loop       1462..1507
                     /label=stem_loop
     misc_feature    1541..1581
                     /label=BBa_B0012
     stem_loop       1548..1567
                     /label=T7 TE
     polyA_site      1568..1581
                     /label=polya
     misc_feature    1575..1575
                     /label=stop
     misc_feature    687..703
                     /label=lac operator
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gagatgctgg atcagcagac cgttgatacc agcaaagcaa ccgttccggt tctgaaagaa
      121 catggtgtta ccattaccac caccttttat cagaacctgt ttgcaaaaca tccggaagtt
      181 cgtccgctgt ttgatatggg tcgtcaggca agcctggaac agccgaaagc actggcaatg
      241 accgttggtg cagcagcaca gaatattgaa aatctgcctg caattctgcc tgccgttcag
      301 aaaattgcag ttaaacattg tcaggcaggc gttgcagcac gtcattatcc gattgttggt
      361 caagaactgc tgggtgcaat taaagagctg ctgggcgacg cagcaaccga tgatattctg
      421 gatgcatggg gtaaagcata tggtgttatt gccgatgttt ttattcaggt tgaagcagat
      481 ctgtatgcac aggatgcaga ataatactag agcaatacgc aaaccgcctc tccccgcgcg
      541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      601 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      661 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag
      721 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
      781 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      841 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      901 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      961 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
     1021 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
     1081 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
     1141 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
     1201 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
     1261 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
     1321 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
     1381 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
     1441 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1501 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1561 gtgggccttt ctgcgtttat a
//