LOCUS       BBa_K1920008             868 bp    DNA     linear       22-Dec-2024
DEFINITION  Pl-RBS-amilCP-TT
ACCESSION   BBa_K1920008
VERSION     BBa_K1920008.1  
FEATURES             Location/Qualifiers
     misc_feature    15..36
                     /label=Modified Consensus Crp binding site
     promoter        1..36
                     /label=BBa_K861170
     misc_feature    49..52
                     /label=conserved
     RBS             45..56
                     /label=BBa_B0034
     CDS             63..728
                     /label=amilCP
     CDS             63..731
                     /label=BBa_K592009
     stem_loop       751..794
                     /label=stem_loop
     misc_feature    740..819
                     /label=BBa_B0010
     terminator      740..819
                     /label=BBa_B0010
     stem_loop       835..854
                     /label=T7 TE
     misc_feature    861..861
                     /label=stop
     polyA_site      855..868
                     /label=polya
     misc_feature    828..868
                     /label=BBa_B0012
     terminator      828..868
                     /label=BBa_B0012
     terminator      740..868
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcaaatgt gattataatc acattttact agagaaagag gagaaatact
       61 agatgagtgt gatcgctaaa caaatgacct acaaggttta tatgtcaggc acggtcaatg
      121 gacactactt tgaggtcgaa ggcgatggaa aaggtaagcc ctacgagggg gagcagacgg
      181 taaagctcac tgtcaccaag ggcggacctc tgccatttgc ttgggatatt ttatcaccac
      241 agtgtcagta cggaagcata ccattcacca agtaccctga agacatccct gactatgtaa
      301 agcagtcatt cccggagggc tatacatggg agaggatcat gaactttgaa gatggtgcag
      361 tgtgtactgt cagcaatgat tccagcatcc aaggcaactg tttcatctac catgtcaagt
      421 tctctggttt gaactttcct cccaatggac ctgtcatgca gaagaagaca cagggctggg
      481 aacccaacac tgagcgtctc tttgcacgag atggaatgct gctaggaaac aactttatgg
      541 ctctgaagtt agaaggaggc ggtcactatt tgtgtgaatt taaaactact tacaaggcaa
      601 agaagcctgt gaagatgcca gggtatcact atgttgaccg caaactggat gtaaccaatc
      661 acaacaagga ttacacttcg gttgagcagt gtgaaatttc cattgcacgc aaacctgtgg
      721 tcgcctaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      781 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      841 ccttcgggtg ggcctttctg cgtttata
//