BBa_K1921006 1 BBa_K1921006 GCW51 2016-09-30T11:00:00Z 2016-10-19T11:21:56Z This part was gained from Pichia pastoris GS115. As a cell wall protein of Pichia pastoris, GCW51 is often used as an anchor protein in Pichia pastoris surface display system because of its stable connection with the dextranomer in cell wall.?? By fusing GCW51 with the target protein expression, the target protein can be displayed on the outer of the yeast cell wall.?? By expressing the fusion protein of GCW51 protein and PETase, PETase was expressed on the surface of Pichia pastoris, and the whole cell catalyst for the degradation of PET was obtained. We made the PETase fixed on the cell wall, which can improve the stability of PETase, and is easy to control the degradation reaction of PET and PETase recycling. false false _2388_ 29455 29455 9 false false Zhuozhi Chen BBa_K1921006_sequence 1 gatgacgatgactcattaccttttgttattgttaactccgcgtcaggagaaacccatgatggaacttactggggtgtgaataacgccggagcagtcgttccaagctccactggagtgcgatttgtggtcaacgacgacggtgagttggaaggtaatgacgaggaagttgaagtgacctcaaacgggtttttgaccttaagagatgacaatgatgaaaatgaggggttttctctcacagatgatgatccaccggttttgctgttaaatcgtcaaactcctaccgtgtggatatgtggatctgatgatgacgcccgtattgctctgggttcacaatcgccacaagatgattgtgtagagtactccattgaagttcagctgcaaagtggttcaagaagcgggtccagcacaagaacaagcagtagaacaactggaaccagtgcaaccagtgcaaccagtgcaaccagtggaaccagtgcaacagggacgactactggaagcacctcgacagctactgatggagcccacaagcttgttggcgggctgtctggattggctggtggtgttgccattgccctattgatctag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z