BBa_K1921021 1 BBa_K1921021 PETase+linker.b+GCW51 2016-10-13T11:00:00Z 2016-10-20T01:31:23Z false false _2388_ 29455 29455 9 false false Zhuozhi Chen component2524999 1 BBa_K1921031 component2524998 1 BBa_K1921000 component2525000 1 BBa_K1921006 annotation2525000 1 BBa_K1921006 range2525000 1 820 1404 annotation2524999 1 BBa_K1921031 range2524999 1 790 819 annotation2524998 1 BBa_K1921000 range2524998 1 1 789 BBa_K1921006 1 BBa_K1921006 GCW51 2016-09-30T11:00:00Z 2016-10-19T11:21:56Z This part was gained from Pichia pastoris GS115. As a cell wall protein of Pichia pastoris, GCW51 is often used as an anchor protein in Pichia pastoris surface display system because of its stable connection with the dextranomer in cell wall.?? By fusing GCW51 with the target protein expression, the target protein can be displayed on the outer of the yeast cell wall.?? By expressing the fusion protein of GCW51 protein and PETase, PETase was expressed on the surface of Pichia pastoris, and the whole cell catalyst for the degradation of PET was obtained. We made the PETase fixed on the cell wall, which can improve the stability of PETase, and is easy to control the degradation reaction of PET and PETase recycling. false false _2388_ 29455 29455 9 false false Zhuozhi Chen BBa_K1921031 1 BBa_K1921031 linker.b 2016-10-16T11:00:00Z 2016-10-17T12:45:33Z false false _2388_ 29455 29455 9 false false Zhuozhi Chen BBa_K1921000 1 BBa_K1921000 PETase 2016-07-18T11:00:00Z 2016-10-19T10:15:22Z Ideonella sakaiensis,sequence found through Genebank http://www.ncbi.nlm.nih.gov/nuccore/908203770?report=graph&sat=23&from=1351&to=2223 PETase protein coding region.This part,Unlike other PET degrading enzymes,is dedicated to the role of PET degradation. Additionally, PETase has been shown to have a degrading efficiency 120 times greater than alternative enzymes. false false _2388_ 29455 29455 9 Not in stock false none false Zhuozhi Chen annotation2524469 1 PETase coding region range2524469 1 1 789 BBa_K1921006_sequence 1 gatgacgatgactcattaccttttgttattgttaactccgcgtcaggagaaacccatgatggaacttactggggtgtgaataacgccggagcagtcgttccaagctccactggagtgcgatttgtggtcaacgacgacggtgagttggaaggtaatgacgaggaagttgaagtgacctcaaacgggtttttgaccttaagagatgacaatgatgaaaatgaggggttttctctcacagatgatgatccaccggttttgctgttaaatcgtcaaactcctaccgtgtggatatgtggatctgatgatgacgcccgtattgctctgggttcacaatcgccacaagatgattgtgtagagtactccattgaagttcagctgcaaagtggttcaagaagcgggtccagcacaagaacaagcagtagaacaactggaaccagtgcaaccagtgcaaccagtgcaaccagtggaaccagtgcaacagggacgactactggaagcacctcgacagctactgatggagcccacaagcttgttggcgggctgtctggattggctggtggtgttgccattgccctattgatctag BBa_K1921031_sequence 1 ggtggtggaggtagtggaggaggtggtagt BBa_K1921000_sequence 1 caaaccaatccttatgcccgtggtcctaatcctaccgccgcgagcttagaagcaagcgccggtccttttaccgttcgtagctttaccgttagccgcccatcaggttatggtgccggtaccgtttactatccaaccaacgccggtggtaccgttggtgccattgccattgttccaggttacaccgcccgtcaaagcagcattaagtggtggggtccgcgtttggccagccatggttttgttgttatcaccatcgataccaacagcaccttggatcaaccaagcagccgtagcagccaacaaatggccgccttgcgtcaagttgccagcctgaacggtactagcagcagcccaatttacggtaaggttgataccgcccgtatgggtgttatgggttggagcatgggtggtggcggtagcttgattagcgccgccaacaacccaagcttgaaagccgcagcaccacaagccccatgggatagcagcaccaactttagcagcgttaccgttcctaccttgatttttgcctgtgagaacgatagcattgccccagttaacagcagcgccttgccaatttacgatagcatgagccgtaacgccaagcaattcttagaaatcaacggtggtagccatagctgtgccaacagcggtaacagcaaccaagccttgattggtaagaaaggcgttgcctggatgaagcgttttatggataacgatacccgttacagcacctttgcctgtgaaaacccaaacagcacccgtgttagcgattttcgtaccgccaactgtagc BBa_K1921021_sequence 1 caaaccaatccttatgcccgtggtcctaatcctaccgccgcgagcttagaagcaagcgccggtccttttaccgttcgtagctttaccgttagccgcccatcaggttatggtgccggtaccgtttactatccaaccaacgccggtggtaccgttggtgccattgccattgttccaggttacaccgcccgtcaaagcagcattaagtggtggggtccgcgtttggccagccatggttttgttgttatcaccatcgataccaacagcaccttggatcaaccaagcagccgtagcagccaacaaatggccgccttgcgtcaagttgccagcctgaacggtactagcagcagcccaatttacggtaaggttgataccgcccgtatgggtgttatgggttggagcatgggtggtggcggtagcttgattagcgccgccaacaacccaagcttgaaagccgcagcaccacaagccccatgggatagcagcaccaactttagcagcgttaccgttcctaccttgatttttgcctgtgagaacgatagcattgccccagttaacagcagcgccttgccaatttacgatagcatgagccgtaacgccaagcaattcttagaaatcaacggtggtagccatagctgtgccaacagcggtaacagcaaccaagccttgattggtaagaaaggcgttgcctggatgaagcgttttatggataacgatacccgttacagcacctttgcctgtgaaaacccaaacagcacccgtgttagcgattttcgtaccgccaactgtagcggtggtggaggtagtggaggaggtggtagtgatgacgatgactcattaccttttgttattgttaactccgcgtcaggagaaacccatgatggaacttactggggtgtgaataacgccggagcagtcgttccaagctccactggagtgcgatttgtggtcaacgacgacggtgagttggaaggtaatgacgaggaagttgaagtgacctcaaacgggtttttgaccttaagagatgacaatgatgaaaatgaggggttttctctcacagatgatgatccaccggttttgctgttaaatcgtcaaactcctaccgtgtggatatgtggatctgatgatgacgcccgtattgctctgggttcacaatcgccacaagatgattgtgtagagtactccattgaagttcagctgcaaagtggttcaagaagcgggtccagcacaagaacaagcagtagaacaactggaaccagtgcaaccagtgcaaccagtgcaaccagtggaaccagtgcaacagggacgactactggaagcacctcgacagctactgatggagcccacaagcttgttggcgggctgtctggattggctggtggtgttgccattgccctattgatctag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z