BBa_K1921006
1
BBa_K1921006
GCW51
2016-09-30T11:00:00Z
2016-10-19T11:21:56Z
This part was gained from Pichia pastoris GS115.
As a cell wall protein of Pichia pastoris, GCW51 is often used as an anchor protein in Pichia pastoris surface display system because of its stable connection with the dextranomer in cell wall.?? By fusing GCW51 with the target protein expression, the target protein can be displayed on the outer of the yeast cell wall.??
By expressing the fusion protein of GCW51 protein and PETase, PETase was expressed on the surface of Pichia pastoris, and the whole cell catalyst for the degradation of PET was obtained. We made the PETase fixed on the cell wall, which can improve the stability of PETase, and is easy to control the degradation reaction of PET and PETase recycling.
false
false
_2388_
29455
29455
9
false
false
Zhuozhi Chen
BBa_K1921031
1
BBa_K1921031
linker.b
2016-10-16T11:00:00Z
2016-10-17T12:45:33Z
false
false
_2388_
29455
29455
9
false
false
Zhuozhi Chen
BBa_K1921000
1
BBa_K1921000
PETase
2016-07-18T11:00:00Z
2016-10-19T10:15:22Z
Ideonella sakaiensis,sequence found through Genebank http://www.ncbi.nlm.nih.gov/nuccore/908203770?report=graph&sat=23&from=1351&to=2223
PETase protein coding region.This part,Unlike other PET degrading enzymes,is dedicated to the role of PET degradation. Additionally, PETase has been shown to have a degrading efficiency 120 times greater than alternative enzymes.
false
false
_2388_
29455
29455
9
Not in stock
false
none
false
Zhuozhi Chen
annotation2524469
1
PETase coding region
range2524469
1
1
789
BBa_K1921021
1
BBa_K1921021
PETase+linker.b+GCW51
2016-10-13T11:00:00Z
2016-10-20T01:31:23Z
false
false
_2388_
29455
29455
9
false
false
Zhuozhi Chen
component2524999
1
BBa_K1921031
component2525000
1
BBa_K1921006
component2524998
1
BBa_K1921000
annotation2524999
1
BBa_K1921031
range2524999
1
790
819
annotation2524998
1
BBa_K1921000
range2524998
1
1
789
annotation2525000
1
BBa_K1921006
range2525000
1
820
1404
BBa_K1921006_sequence
1
gatgacgatgactcattaccttttgttattgttaactccgcgtcaggagaaacccatgatggaacttactggggtgtgaataacgccggagcagtcgttccaagctccactggagtgcgatttgtggtcaacgacgacggtgagttggaaggtaatgacgaggaagttgaagtgacctcaaacgggtttttgaccttaagagatgacaatgatgaaaatgaggggttttctctcacagatgatgatccaccggttttgctgttaaatcgtcaaactcctaccgtgtggatatgtggatctgatgatgacgcccgtattgctctgggttcacaatcgccacaagatgattgtgtagagtactccattgaagttcagctgcaaagtggttcaagaagcgggtccagcacaagaacaagcagtagaacaactggaaccagtgcaaccagtgcaaccagtgcaaccagtggaaccagtgcaacagggacgactactggaagcacctcgacagctactgatggagcccacaagcttgttggcgggctgtctggattggctggtggtgttgccattgccctattgatctag
BBa_K1921031_sequence
1
ggtggtggaggtagtggaggaggtggtagt
BBa_K1921000_sequence
1
caaaccaatccttatgcccgtggtcctaatcctaccgccgcgagcttagaagcaagcgccggtccttttaccgttcgtagctttaccgttagccgcccatcaggttatggtgccggtaccgtttactatccaaccaacgccggtggtaccgttggtgccattgccattgttccaggttacaccgcccgtcaaagcagcattaagtggtggggtccgcgtttggccagccatggttttgttgttatcaccatcgataccaacagcaccttggatcaaccaagcagccgtagcagccaacaaatggccgccttgcgtcaagttgccagcctgaacggtactagcagcagcccaatttacggtaaggttgataccgcccgtatgggtgttatgggttggagcatgggtggtggcggtagcttgattagcgccgccaacaacccaagcttgaaagccgcagcaccacaagccccatgggatagcagcaccaactttagcagcgttaccgttcctaccttgatttttgcctgtgagaacgatagcattgccccagttaacagcagcgccttgccaatttacgatagcatgagccgtaacgccaagcaattcttagaaatcaacggtggtagccatagctgtgccaacagcggtaacagcaaccaagccttgattggtaagaaaggcgttgcctggatgaagcgttttatggataacgatacccgttacagcacctttgcctgtgaaaacccaaacagcacccgtgttagcgattttcgtaccgccaactgtagc
BBa_K1921021_sequence
1
caaaccaatccttatgcccgtggtcctaatcctaccgccgcgagcttagaagcaagcgccggtccttttaccgttcgtagctttaccgttagccgcccatcaggttatggtgccggtaccgtttactatccaaccaacgccggtggtaccgttggtgccattgccattgttccaggttacaccgcccgtcaaagcagcattaagtggtggggtccgcgtttggccagccatggttttgttgttatcaccatcgataccaacagcaccttggatcaaccaagcagccgtagcagccaacaaatggccgccttgcgtcaagttgccagcctgaacggtactagcagcagcccaatttacggtaaggttgataccgcccgtatgggtgttatgggttggagcatgggtggtggcggtagcttgattagcgccgccaacaacccaagcttgaaagccgcagcaccacaagccccatgggatagcagcaccaactttagcagcgttaccgttcctaccttgatttttgcctgtgagaacgatagcattgccccagttaacagcagcgccttgccaatttacgatagcatgagccgtaacgccaagcaattcttagaaatcaacggtggtagccatagctgtgccaacagcggtaacagcaaccaagccttgattggtaagaaaggcgttgcctggatgaagcgttttatggataacgatacccgttacagcacctttgcctgtgaaaacccaaacagcacccgtgttagcgattttcgtaccgccaactgtagcggtggtggaggtagtggaggaggtggtagtgatgacgatgactcattaccttttgttattgttaactccgcgtcaggagaaacccatgatggaacttactggggtgtgaataacgccggagcagtcgttccaagctccactggagtgcgatttgtggtcaacgacgacggtgagttggaaggtaatgacgaggaagttgaagtgacctcaaacgggtttttgaccttaagagatgacaatgatgaaaatgaggggttttctctcacagatgatgatccaccggttttgctgttaaatcgtcaaactcctaccgtgtggatatgtggatctgatgatgacgcccgtattgctctgggttcacaatcgccacaagatgattgtgtagagtactccattgaagttcagctgcaaagtggttcaagaagcgggtccagcacaagaacaagcagtagaacaactggaaccagtgcaaccagtgcaaccagtgcaaccagtggaaccagtgcaacagggacgactactggaagcacctcgacagctactgatggagcccacaagcttgttggcgggctgtctggattggctggtggtgttgccattgccctattgatctag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z