LOCUS BBa_K1923006 5047 bp DNA linear 16-Feb-2025 DEFINITION NLS-FLAG-EGFP-dCas9-NLS encoding gene ACCESSION BBa_K1923006 VERSION BBa_K1923006.1 FEATURES Location/Qualifiers CDS 58..79 /label=Nucleus localization sequence encoding gene CDS 80..146 /label=Triple FLAG tag encoding gene CDS 151..867 /label=EGFP encoding gene CDS 868..4978 /label=dCas9 encoding gene CDS 4979..5000 /label=Nucleus localization sequence encoding gene ORIGIN 1 gggagtactt gtttttagaa tatacggtca acgaactata attaactaaa gatggctcca 61 aagaagaagc gtaaggtaga ctacaaagac catgacggtg attataaaga tcatgacatc 121 gattacaagg atgacgatga caagctcgag atggtgagca agggcgagga gctgttcacc 181 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 241 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 301 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 361 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 421 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 481 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 541 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 601 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 661 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 721 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 781 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 841 actctcggca tggacgagct gtacaaggtc gagatggata agaaatactc aataggctta 901 gctatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct 961 aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg 1021 gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt 1081 agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag 1141 atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa 1201 gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat 1261 gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg 1321 gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg 1381 attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta 1441 caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa 1501 gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc 1561 cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc 1621 cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat 1681 acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg 1741 tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat 1801 actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat 1861 caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa 1921 atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa 1981 gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta 2041 ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct 2101 attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt 2161 tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgcattcct 2221 tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct 2281 gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa 2341 tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca 2401 aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat 2461 gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt 2521 gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc 2581 aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct 2641 tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat 2701 gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg 2761 gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa 2821 cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt 2881 attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc 2941 aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa 3001 aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt 3061 agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa 3121 gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca 3181 actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa 3241 gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa 3301 aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat 3361 attaatcgtt taagtgatta tgatgtcgat gccattgttc cacaaagttt ccttaaagac 3421 gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac 3481 gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc 3541 aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt 3601 gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag 3661 catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt 3721 attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat 3781 ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta 3841 aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc 3901 tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata 3961 ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa 4021 attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact 4081 ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg 4141 ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca 4201 attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa 4261 aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg 4321 gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg 4381 gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa 4441 gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt 4501 cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca 4561 agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca 4621 gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt 4681 attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa 4741 gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt 4801 attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca 4861 acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat 4921 caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgaccca 4981 aagaagaagc gtaaggtatg actcatgtaa ttagttatgt cacgcttaca ttcacgccct 5041 cctccta //