LOCUS BBa_K1923007 5797 bp DNA linear 22-Dec-2024 DEFINITION Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene ACCESSION BBa_K1923007 VERSION BBa_K1923007.1 FEATURES Location/Qualifiers CDS 3..444 /label=Gal4 binding domain CDS 481..502 /label=Nucleus localization sequence CDS 503..569 /label=Triple FLAG tag CDS 576..1295 /label=EGFP encoding gene CDS 1296..5400 /label=dCas9 encoding gene CDS 5401..5422 /label=Nucleus localization sequence CDS 5445..5796 /label=Gal4 activating domain ORIGIN 1 tgatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca 61 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct 121 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa 181 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga 241 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag 301 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc 361 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag 421 gtcaaagaca gttgactgta tcgtcgagga gcctgggcag cggctccggg atcatggctc 481 caaagaagaa gcgtaaggta gactacaaag accatgacgg tgattataaa gatcatgaca 541 tcgattacaa ggatgacgat gacaagctcg agatggtgag caagggcgag gagctgttca 601 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 661 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 721 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 781 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 841 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 901 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 961 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 1021 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 1081 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 1141 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 1201 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 1261 tcactctcgg catggacgag ctgtacaagg tcgagatgga taagaaatac tcaataggct 1321 tagctatcgg cacaaatagc gtcggatggg cggtgatcac tgatgaatat aaggttccgt 1381 ctaaaaagtt caaggttctg ggaaatacag accgccacag tatcaaaaaa aatcttatag 1441 gggctctttt atttgacagt ggagagacag cggaagcgac tcgtctcaaa cggacagctc 1501 gtagaaggta tacacgtcgg aagaatcgta tttgttatct acaggagatt ttttcaaatg 1561 agatggcgaa agtagatgat agtttctttc atcgacttga agagtctttt ttggtggaag 1621 aagacaagaa gcatgaacgt catcctattt ttggaaatat agtagatgaa gttgcttatc 1681 atgagaaata tccaactatc tatcatctgc gaaaaaaatt ggtagattct actgataaag 1741 cggatttgcg cttaatctat ttggccttag cgcatatgat taagtttcgt ggtcattttt 1801 tgattgaggg agatttaaat cctgataata gtgatgtgga caaactattt atccagttgg 1861 tacaaaccta caatcaatta tttgaagaaa accctattaa cgcaagtgga gtagatgcta 1921 aagcgattct ttctgcacga ttgagtaaat caagacgatt agaaaatctc attgctcagc 1981 tccccggtga gaagaaaaat ggcttatttg ggaatctcat tgctttgtca ttgggtttga 2041 cccctaattt taaatcaaat tttgatttgg cagaagatgc taaattacag ctttcaaaag 2101 atacttacga tgatgattta gataatttat tggcgcaaat tggagatcaa tatgctgatt 2161 tgtttttggc agctaagaat ttatcagatg ctattttact ttcagatatc ctaagagtaa 2221 atactgaaat aactaaggct cccctatcag cttcaatgat taaacgctac gatgaacatc 2281 atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa aagtataaag 2341 aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg ggagctagcc 2401 aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt actgaggaat 2461 tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt gacaacggct 2521 ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga caagaagact 2581 tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact tttcgcattc 2641 cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg actcggaagt 2701 ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt gcttcagctc 2761 aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa aaagtactac 2821 caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca aaggtcaaat 2881 atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag aaagccattg 2941 ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa gaagattatt 3001 tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat agatttaatg 3061 cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat tttttggata 3121 atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta tttgaagata 3181 gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat aaggtgatga 3241 aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa ttgattaatg 3301 gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca gatggttttg 3361 ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa gaagacattc 3421 aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca aatttagctg 3481 gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat gaattggtca 3541 aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt gaaaatcaga 3601 caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca 3661 aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa ttgcaaaatg 3721 aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac caagaattag 3781 atattaatcg tttaagtgat tatgatgtcg atgccattgt tccacaaagt ttccttaaag 3841 acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt aaatcggata 3901 acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg 3961 ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt ggaggtttga 4021 gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc caaatcacta 4081 agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa aatgataaac 4141 ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac ttccgaaaag 4201 atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat gatgcgtatc 4261 taaatgccgt cgttggaact gctttgatta agaaatatcc aaaacttgaa tcggagtttg 4321 tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa 4381 taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc ttcaaaacag 4441 aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact aatggggaaa 4501 ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa gtattgtcca 4561 tgccccaagt caatattgtc aagaaaacag aagtacagac aggcggattc tccaaggagt 4621 caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac tgggatccaa 4681 aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg gttgctaagg 4741 tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg atcacaatta 4801 tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa ggatataagg 4861 aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag ttagaaaacg 4921 gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag ctggctctgc 4981 caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg aagggtagtc 5041 cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat ttagatgaga 5101 ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc aatttagata 5161 aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa gcagaaaata 5221 ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa tattttgata 5281 caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc actcttatcc 5341 atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta ggaggtgacc 5401 caaagaagaa gcgtaaggta tccctcagcc tgggcagcgg ctccgccaat tttaatcaaa 5461 gtgggaatat tgctgatagc tcattgtcct tcactttcac taacagtagc aacggtccga 5521 acctcataac aactcaaaca aattctcaag cgctttcaca accaattgcc tcctctaacg 5581 ttcatgataa cttcatgaat aatgaaatca cggctagtaa aattgatgat ggtaataatt 5641 caaaaccact gtcacctggt tggacggacc aaactgcgta taacgcgttt ggaatcacta 5701 cagggatgtt taataccact acaatggatg atgtatataa ctatctattc gatgatgaag 5761 ataccccacc aaacccaaaa aaagagggtg ggtagta //