BBa_K1925004 1 U6 array 3xtetO-U6 tandom repeat(U6 array) 2016-10-10T11:00:00Z 2016-10-11T08:02:44Z Our iGEM team built it Three U6 tandom series with restricition enzyme site behind each tetO-U6,which makes it possible to drive three independent genes of interest respectively.For us,we can use it to drive three different shRNAs simutaneously. false false _2392_ 29381 29381 9 false Use proper restriction enzymes and design proper enzyme site behind and before your gene of interest false Zi Yue Wang annotation2492362 1 tetO U6 range2492362 1 379 716 annotation2492364 1 tetO U6 range2492364 1 757 1094 annotation2492361 1 Hind III site range2492361 1 339 378 annotation2492360 1 tetO U6 range2492360 1 1 338 annotation2492363 1 Nhe I site range2492363 1 717 756 BBa_K1925004_sequence 1 acttgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaagctttgccctggcccaccactagagtatcttaggatccacttgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgagctagcctacggcgtgcagtactagagtatcttaggatccacttgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z