BBa_K1925005 1 BBa_K1925005 3xtRNA tandom repeat(tRNA array) 2016-10-10T11:00:00Z 2016-10-11T08:33:46Z De novo synthesis by company Genewiz In order to express three shRNAs simutaneously within only one transcript,we design design artificial tandom series tRNAs to imitate natural tandom series tRNAs,in which we design restriction enzyme sites between each tRNAs.Using these restriction enzyme sites we can easily insert shRNA false false _2392_ 29381 29381 9 false Cuz it has many tandom repeat sequences,PCR the sequence is difficult. false Zi Yue Wang annotation2492512 1 Sal I range2492512 1 280 285 annotation2492511 1 tRNA range2492511 1 203 279 annotation2492513 1 tRNA range2492513 1 286 362 annotation2492507 1 tRNA range2492507 1 36 111 annotation2492509 1 tRNA range2492509 1 118 194 annotation2492510 1 Not I range2492510 1 195 202 annotation2492514 1 Hind III range2492514 1 373 378 annotation2492506 1 AgeI range2492506 1 27 35 annotation2492508 1 NcoI range2492508 1 112 117 BBa_K1925005_sequence 1 atatcttgtggaaaggacgaactagagtaaccggtaacaaagcaccagtggtctagtggtagaatagtaccctgccacggtacagacccgggttcgattcccggctggtgcccatggaacaaagcaccagtggtctagtggtagaatagtaccctgccacggtacagacccgggttcgattcccggctggtgcagcggccgcaacaaagcaccagtggtctagtggtagaatagtaccctgccacggtacagacccgggttcgattcccggctggtgcagtcgacaacaaagcaccagtggtctagtggtagaatagtaccctgccacggtacagacccgggttcgattcccggctggtgcattttttttttaagctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z