BBa_K1925006 1 BBa_K1925006 mitronic GFP backbone 2016-10-10T11:00:00Z 2016-10-11T09:15:53Z De novo synthesis from company Genewiz In order to express three shRNAs simutaneously within only one transcript,we design design artificial introns within GFP's CDS,in which we design restriction enzyme sites between each exons.Using these restriction enzyme sites we can easily insert shRNAs. false false _2392_ 29381 29381 9 false Cuz its complexity of sequence，PCR will be a little difficult false Zi Yue Wang annotation2492562 1 GFP exon 2 range2492562 1 209 442 annotation2492566 1 GFP exon 4 range2492566 1 684 827 annotation2492564 1 GFP exon 3 range2492564 1 479 649 annotation2492560 1 GFP exon 1 range2492560 1 1 171 annotation2492565 1 intron with Sal I range2492565 1 650 683 annotation2492563 1 intron with Not I range2492563 1 443 478 annotation2492561 1 intron with Age I range2492561 1 172 208 BBa_K1925006_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgcccgtgaggtaaccggtcagttccttctctctctctcaagtggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcgtgaggcggccgccagttccttctctctctctcaagaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggcgtgaggtcgaccagttccttctctctctctcaagcccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z