BBa_K1926011
1
BBa_K1926011
The SNAP UNIT: SNAP-tag flanked by loxP
2016-10-08T11:00:00Z
2016-10-19T01:22:24Z
The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.
This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.
You may use this part to:
1) Localize proteins in vivo with BLOCK and dye from NEB company;
2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein;
3) Fast cut off this part by transient transfecting recombinase gene into nucleus.
false
false
_2393_
28998
28998
9
false
None
false
Xinyi Liu
annotation2491776
1
loxP
range2491776
1
755
788
annotation2491779
1
sv40 terminator
range2491779
1
605
754
annotation2491851
1
TAA
range2491851
1
626
628
annotation2491774
1
loxP
range2491774
1
1
34
annotation2491738
1
SNAP-tag
range2491738
1
59
604
annotation2491778
1
NLS
range2491778
1
38
58
BBa_K1926011_sequence
1
ataacttcgtatagcatacattatacgaagttatatgcctaagaagaagaggaaggtcatggacaaagactgcgaaatgaagcgcaccaccctggatagccctctgggcaagctggaactgtctgggtgcgaacagggcctgcaccgtatcatcttcctgggcaaaggaacatctgccgccgacgccgtggaagtgcctgccccagccgccgtgctgggcggaccagagccactgatgcaggccaccgcctggctcaacgcctactttcaccagcctgaggccatcgaggagttccctgtgccagccctgcaccacccagtgttccagcaggagagctttacccgccaggtgctgtggaaactgctgaaagtggtgaagttcggagaggtcatcagctacagccacctggccgccctggccggcaatcccgccgccaccgccgccgtgaaaaccgccctgagcggaaatcccgtgcccattctgatcccctgccaccgggtggtgcagggcgacctggacgtggggggctacgagggcgggctcgccgtgaaagagtggctgctggcccacgagggccacagactgggcaagcctgggctgggtcaacatgttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgaggtaataacttcgtatagcatacattatacgaagttat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z