BBa_K1926012 1 BBa_K1926012 The VIKA UNIT: recombinase VIKA flanked by Vox 2016-10-08T11:00:00Z 2016-10-11T03:47:23Z The recombinase gene VIKA and its RTS Vox were got through denovo synthesis. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdn3.1. This part is the VIKA UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. This part is the improvement of the BBa_K1641003，which is a plain VIKA. You may use this part to: 1) Test the cut-off efficiency of recombinase VIKA by qRT-PCR; 2) Fast cut off this part by transient transfecting recombinase gene into nucleus; 3) Do lineage tracking. false false _2393_ 28998 28998 9 false None false Xinyi Liu annotation2491858 1 TAA range2491858 1 1139 1141 annotation2491855 1 VIKA range2491855 1 35 1117 annotation2491854 1 Vox range2491854 1 1 34 annotation2491856 1 Vox range2491856 1 1261 1294 annotation2491857 1 sv40 terminator range2491857 1 1118 1260 BBa_K1926012_sequence 1 aataggtccgagaatgacagttctcagaaattgaatgaccgatctgactccgtttccaccgttggaacatctggaacctgatgaatttgccgacttagtcagaaaagcgattaagcgcgatccacaagccggtgcgcaccctgcaattcaaagcgcgatcagtcattttcaggacgaatttgtacgtagacaaggcgaatggcaaccagctacattacagcgtttgagaaatgcatggaacgtttttgtgagatggtgtacgcaccaaggtattccggctctgcctgcacgtcatcaggatgttgaacgttatctgatcgaacgccgtaatgaattacaccgcaacaccctgaaagttcatttatgggccattggcaagactcacgtgatcagtggcctgcctaatccatgcgcgcatcgttacgtcaaagcccaaatggcgcagattactcatcaaaaagtacgcgaacgtgaaagaatcgaacaggctcctgcattccgcgaatccgatctggaccgtttaaccgaattgtggtccgctacaagatcggtcacgcaacagcgcgatttgatgattgtttctctggcttatgaaactctgttgcgtaagaacaacctggaacaaatgaaagtgggtgatattgaattttgtcaggacggcagcgccctgattacaatccctttctctaaaacgaaccactcaggtcgcgatgacgtccgttggattagtccacaagtcgctaatcaggtacatgcatacctgcaattacctaacatcgatgccgacccacaatgctttttgctgcaacgtgttaaacgttccggcaaggcgttgaatccagaatcgcataataccctgaacggccatcacccggttagcgaaaaattaattagtagagtgttcgaaagagcttggcgcgcattgaaccacgaaactggtccacgttatactggccattctgcaagagtgggtgctgcacaagatttattgcaggaaggctactcaacactgcaagttatgcaggctggtggctggtcttcagaaaaaatggtgttacgttatggcagacatttgcacgcacatacgtctgctatggctcaaaagagacgccagcgccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgaaataggtccgagaatgacagttctcagaaattga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z