BBa_K1926012
1
BBa_K1926012
The VIKA UNIT: recombinase VIKA flanked by Vox
2016-10-08T11:00:00Z
2016-10-11T03:47:23Z
The recombinase gene VIKA and its RTS Vox were got through denovo synthesis. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdn3.1.
This part is the VIKA UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. This part is the improvement of the BBa_K1641003,which is a plain VIKA.
You may use this part to:
1) Test the cut-off efficiency of recombinase VIKA by qRT-PCR;
2) Fast cut off this part by transient transfecting recombinase gene into nucleus;
3) Do lineage tracking.
false
false
_2393_
28998
28998
9
false
None
false
Xinyi Liu
annotation2491854
1
Vox
range2491854
1
1
34
annotation2491856
1
Vox
range2491856
1
1261
1294
annotation2491858
1
TAA
range2491858
1
1139
1141
annotation2491857
1
sv40 terminator
range2491857
1
1118
1260
annotation2491855
1
VIKA
range2491855
1
35
1117
BBa_K1926012_sequence
1
aataggtccgagaatgacagttctcagaaattgaatgaccgatctgactccgtttccaccgttggaacatctggaacctgatgaatttgccgacttagtcagaaaagcgattaagcgcgatccacaagccggtgcgcaccctgcaattcaaagcgcgatcagtcattttcaggacgaatttgtacgtagacaaggcgaatggcaaccagctacattacagcgtttgagaaatgcatggaacgtttttgtgagatggtgtacgcaccaaggtattccggctctgcctgcacgtcatcaggatgttgaacgttatctgatcgaacgccgtaatgaattacaccgcaacaccctgaaagttcatttatgggccattggcaagactcacgtgatcagtggcctgcctaatccatgcgcgcatcgttacgtcaaagcccaaatggcgcagattactcatcaaaaagtacgcgaacgtgaaagaatcgaacaggctcctgcattccgcgaatccgatctggaccgtttaaccgaattgtggtccgctacaagatcggtcacgcaacagcgcgatttgatgattgtttctctggcttatgaaactctgttgcgtaagaacaacctggaacaaatgaaagtgggtgatattgaattttgtcaggacggcagcgccctgattacaatccctttctctaaaacgaaccactcaggtcgcgatgacgtccgttggattagtccacaagtcgctaatcaggtacatgcatacctgcaattacctaacatcgatgccgacccacaatgctttttgctgcaacgtgttaaacgttccggcaaggcgttgaatccagaatcgcataataccctgaacggccatcacccggttagcgaaaaattaattagtagagtgttcgaaagagcttggcgcgcattgaaccacgaaactggtccacgttatactggccattctgcaagagtgggtgctgcacaagatttattgcaggaaggctactcaacactgcaagttatgcaggctggtggctggtcttcagaaaaaatggtgttacgttatggcagacatttgcacgcacatacgtctgctatggctcaaaagagacgccagcgccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgaaataggtccgagaatgacagttctcagaaattga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z