BBa_K1929203
1
BBa_K1929203
Her2-DARPin
2016-10-12T11:00:00Z
2016-10-13T03:25:33Z
Designed data online
Anti-Her2 DARPin
false
false
_2396_
26884
26884
9
false
Gastrointestinal oncoproteins
false
Maxwell Ng
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
BBa_K1929202
1
BBa_K1929202
6His tag
2016-10-12T11:00:00Z
2016-10-13T03:23:12Z
Data
6X His tag.
false
false
_2396_
26884
26884
9
false
His tag antibody compatible.
false
Maxwell Ng
BBa_K1929200
1
BBa_K1929200
Anti-Her2 DARPin with Cell-wall-anchor and His6 tag
2016-10-12T11:00:00Z
2016-10-13T03:30:34Z
Designed
Cell wall anchor provides Gram-positive bacteria compatibility. His6 tag acts as reporter. Anti-Her2 DARPin used to bind Her2 oncoprotein (e.g. gastrointestinal cancers, breast cancers).
false
false
_2396_
26884
26884
9
false
Gram-positive compatibility.
false
Maxwell Ng
component2499518
1
BBa_K1929202
component2499517
1
BBa_K1929201
component2499526
1
BBa_B0015
component2499519
1
BBa_K1929203
annotation2499517
1
BBa_K1929201
range2499517
1
1
443
annotation2499518
1
BBa_K1929202
range2499518
1
452
469
annotation2499526
1
BBa_B0015
range2499526
1
888
1016
annotation2499519
1
BBa_K1929203
range2499519
1
478
879
BBa_K1929201
1
BBa_K1929201
acmA1 caw with RBS
2016-10-12T11:00:00Z
2016-10-13T03:26:19Z
Data
Cell wall anchor
false
false
_2396_
26884
26884
9
false
Gram positive
false
Maxwell Ng
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1929200_sequence
1
tccctatcagaggaggtattgacatatgccagtatcacgtgttaaagttaaaaatagacatttaaaaaagaaaactaaaaaaccactcgctttttataaaccagccacaaaatttgctggcgctgttcttattgccggaacattgacaaccacacatgaacttcttcttcaacagacaagtccaatggttcaagcagacggagcttcttcagctggaaatactaattctggtggctcgacaaccacaattacgaataataattctggaaccaatagcagttcaactacttataccgtcaaatctggtgatactctttggggaatctcacaaagatatggaattagtgtcgctcaaattcaaagtgcgaataatcttaaaagtaccattatctacattggtcaaaaacttgtactgacaggttcagcttcttctacaaattcagtactagagcatcaccatcaccatcactactagagcatcaccatcaccatcactcacggggctccgacctgggcaaaaagctgctggaggccgctagggccgggcaggacgatgaagtgagaatcctgatggccaacggggctgacgtgaatgctaaggatgagtacggcctgacccccctgtatctggctacagcacacggccatctggagatcgtggaagtcctgctgaaaaacggagccgacgtgaatgcagtcgatgccattgggttcactcctctgcacctggcagcctttatcggacatctggagattgcagaagtgctgctgaagcacggcgctgacgtgaacgcacaggataagttcggaaaaaccgcttttgacatcagcattggcaacggaaatgaagacctggctgaaatcctgcagaaactgaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1929203_sequence
1
catcaccatcaccatcactcacggggctccgacctgggcaaaaagctgctggaggccgctagggccgggcaggacgatgaagtgagaatcctgatggccaacggggctgacgtgaatgctaaggatgagtacggcctgacccccctgtatctggctacagcacacggccatctggagatcgtggaagtcctgctgaaaaacggagccgacgtgaatgcagtcgatgccattgggttcactcctctgcacctggcagcctttatcggacatctggagattgcagaagtgctgctgaagcacggcgctgacgtgaacgcacaggataagttcggaaaaaccgcttttgacatcagcattggcaacggaaatgaagacctggctgaaatcctgcagaaactgaat
BBa_K1929201_sequence
1
tccctatcagaggaggtattgacatatgccagtatcacgtgttaaagttaaaaatagacatttaaaaaagaaaactaaaaaaccactcgctttttataaaccagccacaaaatttgctggcgctgttcttattgccggaacattgacaaccacacatgaacttcttcttcaacagacaagtccaatggttcaagcagacggagcttcttcagctggaaatactaattctggtggctcgacaaccacaattacgaataataattctggaaccaatagcagttcaactacttataccgtcaaatctggtgatactctttggggaatctcacaaagatatggaattagtgtcgctcaaattcaaagtgcgaataatcttaaaagtaccattatctacattggtcaaaaacttgtactgacaggttcagcttcttctacaaattcag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1929202_sequence
1
catcaccatcaccatcac
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z