LOCUS BBa_K1929400 3221 bp DNA linear 22-Nov-2024 DEFINITION Las Quorum System ACCESSION BBa_K1929400 VERSION BBa_K1929400.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1647..1678 /label=LVA CDS 1068..1646 /label=luxI misc_feature 1068..1685 /label=BBa_C0061 misc_feature 1686..1710 /label=Help:Barcodes CDS 1068..1685 /label=BBa_C0061 CDS 2371..3081 /label=mCherry misc_feature 2722..2722 /label=C->T (removing PstI site) misc_feature 2371..3084 /label=BBa_J06504 stem_loop 3104..3147 /label=stem_loop misc_feature 3093..3172 /label=BBa_B0010 terminator 3093..3172 /label=BBa_B0010 stem_loop 3188..3207 /label=T7 TE misc_feature 3214..3214 /label=stop polyA_site 3208..3221 /label=polya misc_feature 3181..3221 /label=BBa_B0012 terminator 3181..3221 /label=BBa_B0012 terminator 3093..3221 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagatg actataatga 1081 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc 1141 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat 1201 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg 1261 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc 1321 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt 1381 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac 1441 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa 1501 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag 1561 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg 1621 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt 1681 aataactctg atagtgctag tgtagatctc tactagagtc cctatcagcg atccgcagga 1741 ggtcgagact gcatatgtac aggatgcaac tcctgtcttg cattgcacta agtcttgcac 1801 ttgtcacaaa cagtgcacct acttcaagtt ctacaaagaa aacacagcta caactggagc 1861 atttactgct ggatttacag atgattttga atggaattaa taattacaag aatcccaaac 1921 tcaccaggat gctcacattt aagttttaca tgcccaagaa ggccacagaa ctgaaacatc 1981 ttcagtgtct tgaagaagaa ctcaaacctc tggaggaagt gctaaattta gctcaaagca 2041 aaaactttca cttaagaccc agggacttaa tcagcaatat caacgtaata gttctggaac 2101 taaagggatc tgaaacaaca ttcatgtgtg aatatgctga tgagacagca accattgtag 2161 aatttctgaa cagatggatt accttttgtc aaagcatcat ctcaacactg acttgatacc 2221 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2281 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 2341 gtttatactc gagctgcgtt tatatactag atggtgagca agggcgagga ggataacatg 2401 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac 2461 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag 2521 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc 2581 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg 2641 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg 2701 accgtgaccc aggactcctc cttgcaggac ggcgagttca tctacaaggt gaagctgcgc 2761 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 2821 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 2881 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 2941 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 3001 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 3061 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca 3121 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3181 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //