LOCUS       BBa_K1931016            1376 bp    DNA     linear       22-Nov-2024
DEFINITION  T7-RBS-Nisin C-Terminator
ACCESSION   BBa_K1931016
VERSION     BBa_K1931016.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=T7
     RBS             47..72
                     /label=RBS
     CDS             73..1326
                     /label=NisC
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaacta gagaaagaca
       61 ggaccctact agatgcgtat tatgatgaat aagaagaata tcaagcgcaa cgtcgagaag
      121 ataatcgcac aatgggatga gcgcacccgg aaaaataaag aaaactttga tttcggcgaa
      181 ttgactttat ctacggggtt acccgggatc atacttatgc tggcggagct taagaacaaa
      241 gataactcga aaatttacca gaagaagatc gataattata tcgagtatat agtaagtaaa
      301 ttgtcgacat acggacttct gacaggaagc ttgtacagcg gcgctgctgg aattgcatta
      361 tccatcctgc atttgagaga ggatgatgaa aagtacaaga atttattaga ctctttaaat
      421 cggtacattg agtacttcgt aagagagaag atagagggat ttaacttaga gaatataacg
      481 cccccagact atgatgttat cgagggttta tcaggcatac tttcctatct gttacttatt
      541 aacgacgagc aatacgatga ccttaagata cttatcatca atttcttgtc caaccttact
      601 aaagagaaca acggtttaat aagcttatac attaaatcag agaaccagat gtctcaaagc
      661 gaatcggaaa tgtacccgct gggctgtttg aatatgggac tggcgcatgg tttggccggc
      721 gtggggtgta ttcttgcata cgcgcacatc aagggataca gtaatgaggc gagtctgtct
      781 gcattacaaa agattatatt catctatgag aaatttgagc ttgagcgtaa gaaacaattt
      841 ctgtggaagg acggtcttgt cgcagacgaa cttaaaaagg aaaaagttat tcgggaggcg
      901 tcattcatca gagatgcgtg gtgttatggc gggccgggga taagcctttt atacttatac
      961 ggtggattgg cactggacaa tgattacttt gtagacaagg cagagaaaat tttggagagt
     1021 gctatgcagc gtaaattagg aattgattca tacatgattt gtcatggata ctctggactt
     1081 attgaaatct gctcattgtt caagcggctt cttaatacaa agaagttcga cagttacatg
     1141 gaagaattta atgtcaactc cgaacaaatt cttgaggaat acggcgacga gtcagggaca
     1201 ggattcttag aggggatctc cggatgtatc ctggtgttat cgaagttcga atactcgatc
     1261 aattttacat attggcggca ggcgttactg ttgtttgacg actttttaaa gggaggtaaa
     1321 cgtaaataat actagagaaa aaaaaacccc gcccctgaca gggcggggtt tttttt
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